Prenylated hydroxystilbenes

ABSTRACT

or pharmaceutically acceptable salts or solvates thereof. R1a-R1d, R2-R3 (and R4 where applicable), and A---B can be as defined herein.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application is a continuation of U.S. patent application Ser. No. 16/221,666, filed on Dec. 17, 2018 and issuing as U.S. Pat. No. 10,696,615 on Jun. 30, 2020, which is a continuation of U.S. patent application Ser. No. 14/115,574, filed on Apr. 4, 2014 and issued as U.S. Pat. No. 10,196,335 on Feb. 5, 2019, which is a national stage application of PCT/AU2012/000482, filed on May 4, 2012, which claims the benefit of Australian Patent Application AU2011901663, filed on May 4, 2011, each of which is incorporated herein by reference.

TECHNICAL FIELD

This invention relates to novel prenylated stilbene compounds and to the use of such compounds in the treatment of diseases and medical disorders, for example cancer and skin aging.

BACKGROUND OF THE INVENTION

Propolis, or so called bee glue, is a complex resinous substance collected by worker honey bees (Apis mellifera) from exudates and secretions of young shoots and buds from certain trees and shrubs. It is used by the bees to seal cracks and holes in their hives, and protect against microbial infections.

Propolis is a rich source of bioactive substances, and the medicinal use of propolis dates back to ancient civilizations. Currently, propolis is extensively available as a natural health product, and is widely used in cosmetics. However, its modern use in medicine is limited, largely due to the wide variations in chemical compositions arising from honey bees collecting from different or a mixture of plant sources. The composition of propolis is dependent upon the surrounding flora to which the bees have access, and as such, differences in flora may result in differences in propolis compositions. For example, it is known that flavonoids are the major pharmacologically active compounds in European propolis, polyprenylated benzophenones are the main substances in Cuban and Venuzuelan propolis, and prenylated cinnamic acid derivatives are predominant in Brazilian propolis.

Recognition of the botanical origin of the propolis produced by honey bees enables beehives to be placed in favourable locations such that propolis from a single botanical source may be produced to enable manufacture of medicines of high quality and efficacy.

The medicinal uniqueness of propolis is determined by the selective collecting ability of honey bees, as they can recognise natural materials that are relatively non-polar and have antibiotic properties. As reported, the common source of propolis is leaf and flower bud exudates, which are of high antibiotic character in order to protect the delicate growing of plant tissue from attack by microorganisms. It has also been reported that honey bees collect exudates from wounded or diseased plant tissues. Such sources are potentially rich in antibiotic substances produced by plants in response to wounding or attack from insects, microorganisms and viruses.

It is not clear from previous studies whether the bees simply collect a plant material that is known as propolis, or if there is metabolic modification or addition from the bees. However, there does not appear to be evidence of significant amounts of material added from honey bees, or strong evidence for metabolic transformation.

Thus, a better understanding is required regarding the composition of propolis in specific geographical locations to be able to utilize it to its full benefit.

In work leading up to the present invention the inventors have conducted a survey of propolis samples isolated from Kangaroo Island (South Australia), and surprisingly found that unlike other propolis which commonly contain flavonoids as active constituents, Kangaroo Island propolis contain stilbenes, more particularly novel prenylated polyhydroxystilbenes (pPHOS) which are similar in their core structure to resveratrol (pictured below).

Resveratrol is the constituent in red wine thought to contribute to the low incidence of heart attack amongst the French despite high consumption of very rich food. In fact, Resveratrol has been shown to inhibit LDL oxidation which leads to atherosclerosis and coronary heart disease. Resveratrol is also a lead compound in preventative anti-ageing medicine, and has also been found to possess anti-cancer and antioxidant activities.

However, despite its therapeutic properties, resveratrol exhibits very poor oral bioavailability and is rapidly metabolized in the intestines and liver into glucuronate and sulfate conjugates. The in vivo effectiveness of resveratrol is only observed at high concentrations (up to 5 g), or in the case of direct administration such as intravenous or local application.

Recently, GlaxoSmithKline (GSK) Pharmaceutical conducted phase IIa clinical trials of its proprietary formulation of resveratrol (3-5 g), SRT501 in the treatment of multiple myeloma; however, the trials were halted as the formulation only offered minimal efficacy, and several patients in the trial developed kidney failure.

Prenylated hydroxystilbenes have been reported in plants, mainly from the Moraceae (Mulberry family, including Morus alba (mulberry), and various Artocarpus species) and Fabaceae (Legume family, including Arachis hypogaea (peanut)) families. The isolated compounds have reportedly exhibited anti-inflammatory, antimicrobial and antioxidant activities, thus suggesting that the prenyl group does not adversely affect the biological activities of hydroxystilbenes.

In fact, prenylated hydroxystilbenes could circumvent the problem of low bioavailability of hydroxystilbenes, such as resveratrol, due to their lipophilic nature. Owing to the presence of one or more prenyl groups, the compounds may be able to cross cell membranes more readily and the formation of glucuronate and sulfate conjugates may be avoided, thus improving the bioavailability of the compounds, and more generally presenting superior drug candidates for the development of new therapeutic agents. Previously, it has been demonstrated that a tetramethoxy derivative of resveratrol was able to cross the blood-brain barrier in rats more easily than resveratrol.

Thus, the novel pPHOS derivatives isolated from the propolis samples of Kangaroo Island are strong candidates for the development of new therapeutic agents in the treatment of diseases such as cancer.

Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is not to be taken as an admission that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application.

SUMMARY

In work leading up to the present invention the inventors have isolated and synthesised a number of novel prenylated polyhydroxystilbenes (pPHOS) derivatives, and carried out in vitro experiments in which these novel compounds have exhibited high potency and selectivity in a panel of cancer cell lines, particularly in leukemia and melanoma cell lines. In other preliminary experiments, a number of the novel pPHOS derivatives were found to modulate activity of the SIRT1 enzyme which indicate that the novel pPHOS compounds of the present invention may be lead therapeutic agents for the treatment of a range of diseases.

Accordingly, in a first aspect of the invention, there is provided a method for treating cancer comprising administering a therapeutically effective amount of a compound of formula (I),

-   -   wherein:     -   R¹ is independently H, OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or         OCH═CHC(CH₃)═CH₂, wherein at least one of R¹ is CH₂CH═C(CH₃)₂,         OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂,         or OCH═CHC(CH₃)═CH₂;     -   R² is selected from OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or         OCH═CHC(CH₃)═CH₂;     -   R³ is selected from H, OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or         OCH═CHC(CH₃)═CH₂;     -   R⁴ is selected from, methyl, ethyl, propyl, isopropyl, butyl,         isobutyl, t-butyl or benzyl;     -   n is an integer selected from the group consisting of 1, 2, 3 or         4;     -   and A----B is selected from CH═CH, CH₂—CH₂, CH═CHX, or CH₂—CH₂X,         where X═(CH₂)pCH₂, and p is an integer selected from the group         consisting of 0, 1, 2 or 3; provided that when R² is OH, R³ is H         or OH, A----B is CH═CH, n is 3 and two of the R¹ groups are OH,         then the third R¹ group is not CH═CHCH(CH₃)₂;     -   or a pharmaceutically acceptable salt, solvate, or         pharmaceutical composition including said compounds, to a         patient in need thereof.

In a second aspect of the invention, there is provided a method for treating cancer comprising administering a therapeutically effective amount of a compound of formula (I),

-   -   wherein:     -   R¹ is independently H, OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or         OCH═CHC(CH₃)═CH₂, wherein at least one of R¹ is CH₂CH═C(CH₃)₂,         OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂,         or OCH═CHC(CH₃)═CH₂;     -   R² is selected from OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or         OCH═CHC(CH₃)═CH₂;     -   R³ is selected from H, OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or         OCH═CHC(CH₃)═CH₂;     -   R⁴ is selected from, methyl, ethyl, propyl, isopropyl, butyl,         isobutyl, t-butyl or benzyl;     -   n is an integer selected from the group consisting of 1, 2, 3 or         4;     -   and A----B is selected from CH═CH, CH₂—CH₂, CH═CHX, or CH₂—CH₂X,         where X═(CH₂)pCH₂, and p is an integer selected from the group         consisting of 0, 1, 2 or 3;     -   or a pharmaceutically acceptable salt, solvate, or         pharmaceutical composition including said compounds, to a         patient in need thereof.

In a third aspect, there is provided a compound of formula (I) according to the first or second aspects, or pharmaceutical composition thereof, for use as a medicament.

In a fourth aspect, there is provided a compound of formula (I) according to the first or second aspects, or pharmaceutical composition thereof, for use in therapy.

In a fifth aspect of the invention there is provided a compound of formula (I) according to the first or second aspects, or pharmaceutical composition thereof, for use in the treatment of cancer.

In a sixth aspect of the invention, there is provided a method for treating immunosuppression comprising administering a therapeutically effective amount of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition including said compounds to a patient in need thereof.

In a seventh aspect of the invention, there is provided a method for treating inflammation comprising administering a therapeutically effective amount of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition including said compounds to a patient in need thereof.

In an eighth aspect of the invention, there is provided a method for treating a bacterial or fungal infection comprising administering a therapeutically effective amount of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition including said compounds to a patient in need thereof.

In a ninth aspect of the invention, there is provided a method for treating skin aging comprising administering a therapeutically effective amount of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition including said compounds to a patient in need thereof.

In a tenth aspect of the invention, there is provided a use of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition thereof in the preparation of a medicament for the treatment of cancer.

In an eleventh aspect of the invention, there is provided a use of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition thereof in the preparation of a medicament for the treatment of immunosuppression.

In a twelfth aspect of the invention, there is provided a use of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition thereof in the preparation of a medicament for the treatment of inflammation.

In a thirteenth aspect of the invention, there is provided a use of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition thereof in the preparation of a medicament for the treatment of a bacterial or fungal infection.

In a fourteenth aspect of the invention, there is provided a use of a compound of formula (I) according to the first or second aspects, or a pharmaceutical composition thereof in the preparation of a medicament for the treatment of skin aging.

In further aspects of the invention, there is provided combinations comprising a compound or pharmaceutical composition as defined above, and at least one further therapeutic agent for the methods and uses as described herein.

According to a fifteenth aspect of the invention, there is provided a compound of formula (Ia)

-   -   or a pharmaceutically acceptable salt or solvate thereof,         wherein:     -   R^(1a), R^(1b), R^(1c) and R^(1d) are each independently H, OH,         OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂,         OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂, wherein at least one of         R^(1a-1d) is CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂,         OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R² is selected from OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R³ is selected from H, OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂,         CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R⁴ is selected from, methyl, ethyl, propyl, isopropyl, butyl,         isobutyl, t-butyl or benzyl;     -   and A----B is selected from CH═CH, CH₂—CH₂, CH═CHX, or CH₂—CH₂X,         where X═(CH₂)pCH₂, and p is an integer selected from the group         consisting of 0, 1, 2 or 3; provided that:     -   (i) when R³ is H and R² is OH or OCH₂CH═C(CH₃)₂, then R^(1b) and         R^(1d) are independently not OH or OCH₂CH═C(CH₃)₂;     -   (ii) when R³ is H, one of R^(1a-1d) is H and at least one of         R^(1a-1d) is CH₂CH═C(CH₃)₂, then A---B is not CH═CH.

In a sixteenth aspect of the invention, there is provided a compound of formula (Ia)

-   -   or a pharmaceutically acceptable salt or solvate thereof,         wherein:     -   R^(1a), R^(1b), R^(1c) and R^(1d) are each independently H, OH,         OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂,         OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂, wherein at least one of         R^(1a-1d) is CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂,         OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R² and R³are each independently selected from OH, OR⁴,         CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂,         or OCH═CHC(CH₃)═CH₂;     -   R⁴ is selected from, methyl, ethyl, propyl, isopropyl, butyl,         isobutyl, t-butyl or benzyl;     -   and A----B is selected from CH═CH, CH₂—CH₂, CH═CHX, or         —CH₂—CH₂X, where X═(CH₂)pCH₂ and p is an integer selected from         the group consisting of 0, 1, 2 or 3.

In one specific example with respect to formula (Ia) or a pharmaceutically acceptable salt or solvate thereof:

R^(1a) is selected from, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;

-   -   R^(1b) is selected from, H, OH, OR⁴, CH₂CH═C(CH₃)₂,         OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂,         or OCH═CHC(CH₃)═CH₂;     -   R^(1c) is selected from H, OH, OR^(4a), CH₂CH═C(CH₃)₂,         OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂,         or OCH═CHC(CH₃)═CH₂;     -   R^(1d) is selected from H, OH, OR⁴, CH₂CH═C(CH₃)₂,         OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂,         or OCH═CHC(CH₃)═CH₂;     -   wherein at least one of R^(1b-1d) is OH and at leat one of         R^(1a-1d) is CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂,         CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R² is selected from OH, OR^(4a), OCH₂CH═C(CH₃)₂,         CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R³ is selected from OH, OR⁴, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂,         OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂;     -   R⁴ is selected from, methyl, ethyl, propyl, isopropyl, butyl,         isobutyl, t-butyl or benzyl;     -   R^(4a) is selected from, ethyl, propyl, isopropyl, butyl,         isobutyl, t-butyl or benzyl; and     -   A----B is selected from CH═CH, CH═CH(CH₂)pCH₂, or         CH₂—CH₂(CH₂)pCH₂, where p is an integer selected from the group         consisting of 1 and 2.

In a seventeenth aspect of the invention, there is provided a pharmaceutical composition comprising a compound of formula (Ia) according to the fifteenth or sixteenth aspects, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable excipient.

According to an eighteenth aspect, there is provided a method for treating cancer, immunosuppression, inflammation, bacterial infection, fungal infection or skin aging comprising administering a therapeutically effective amount of a compound of formula (Ia) according to the fifteenth or sixteenth aspects, or a pharmaceutical composition including said compounds to a patient in need thereof.

In a nineteenth aspect of the invention, there is provided a use of a compound of formula (Ia) according to the fifteenth or sixteenth aspects, or a pharmaceutical composition thereof in the preparation of a medicament for the treatment of cancer, immunosuppression, inflammation, bacterial infection, fungal infection or skin aging.

According to the twentieth aspect of the invention, there is provided a method of preparing compounds of formula (Ib) which comprises the following steps:

-   -   (i) treating the carboxylic acid with a suitable agent to         provide the acid chloride as follows;

-   -   (ii) condensation of the corresponding acid chloride with an         aryl alkene as follows;

-   -   (iii) deprotection of the acetate group and alkylation as         follows;

-   -   wherein, R⁴, R⁵ and R⁶ are each independently selected from the         group OMe, OEt, OPr, O^(i)Pr, OBu, O^(i)Bu, O^(t)Bu, and OBn.

In this and other examples, as is conventionally understood in the context listed, Me is methyl, Et is ethyl, Pr is n-propyl, ^(i)Pr is isopropyl, Bu is n-butyl, ^(i)Bu is isobutyl, ^(t)Bu is tert-butyl, and Bn is benzyl.

In one embodiment, the method comprises the additional step as follows:

-   -   (iv) a hydrogenation reaction as follows:

-   -   wherein, R⁴, R⁵ and R⁶ are as previously defined, provided that         at least one of R⁴, R⁵ or R⁶ is OBn;     -   R⁷, R⁸ and R⁹ are each independently selected from the group         consisting of OH, OMe, OEt, OPr, O^(i)Pr, OBu, O^(i)Bu, and         O^(t)Bu.

In another embodiment the method comprises two additional steps as follows:

-   -   (v) rearrangement of the prenyl group as follows:

-   -   (vi) and a hydrogenation reaction as follows:

-   -   wherein, R⁴, R⁵ and R⁶ are as previously defined, provided that         at least one of R⁴, R⁵ or R⁶ is OBn;     -   R⁷, R⁸ and R⁹ are each independently selected from the group         consisting of OH, OMe, OEt, OPr, O^(i)Pr, Obu, O^(i)Bu, and         O^(t)Bu.

In a twenty first aspect of the invention, there is provided a compound according to USYDS15 as identified below

There is also provided a pharmaceutical composition comprising the compound USYDS15 according to the twenty first aspect, or a pharmaceutically acceptable salt or solvate thereof, and a pharmaceutically acceptable excipient.

In a twenty third aspect of the invention, there is provided use of the compound according to the twenty first aspect or the composition of the twenty second aspect, in the preparation of a medicament for the treatment of cancer, immunosuppresion, inflammation, fungal or bacterial infection, or for the treatment of skin aging.

DETAILED DESCRIPTION

Throughout this specification the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

While it is possible that, for use in therapy a therapeutically effective amount of the compounds as defined herein, or a pharmaceutically acceptable salt or solvate thereof, may be administered as the raw chemical; in one aspect of the present invention the active ingredient is presented as a pharmaceutical composition. Thus, in a further embodiment the invention provides a pharmaceutical composition comprising a compound of formula (I) or (Ia) according to the first, second, fifteenth and sixteenth aspects, or a pharmaceutically acceptable salt or solvate thereof, in admixture with one or more pharmaceutically acceptable carriers, diluents, or excipients. The carrier(s), diluent(s) or excipient(s) must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

When applicable, the compounds of the present invention, including the compounds of formula (I) or (Ia) may be in the form of and/or may be administered as a pharmaceutically acceptable salt.

As used herein the term “pharmaceutically acceptable salt” refers to salts which are toxicologically safe for systemic administration. The pharmaceutically acceptable salts may be selected from alkali or alkaline earth metal salts, including, sodium, lithium, potassium, calcium, magnesium and the like, as well as non-toxic ammonium, quaternary ammonium, or amine cations, including, but not limited to ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, ethylamine, triethanolamine or the like.

As used herein the term “pharmaceutically acceptable excipient” refers to a solid or liquid filler, diluent or encapsulating substance that may be safely used in systemic administration. Depending upon the particular route of administration, a variety of carriers, well known in the art may be used. These carriers or excipients may be selected from a group including sugars, starches, cellulose and its derivatives, malt, gelatine, talc, calcium sulfate, vegetable oils, synthetic oils, polyols, alginic acid, phosphate buffered solutions, emulsifiers, isotonic saline, or pyrogen-free water.

As used herein, the term “solvate” refers to a complex of variable stoichiometry formed by a solute (in this invention, a compound of formula (I) or (Ia) or a salt or physiologically functional derivative thereof) and a solvent. Such solvents for the purpose of the invention may not interfere with the biological activity of the solute. Examples of suitable solvents include, but are not limited to, water, methanol, ethanol and acetic acid. In particular the solvent used is a pharmaceutically acceptable solvent. Examples of suitable pharmaceutically acceptable solvents include, without limitation, water, ethanol, acetic acid, glycerol, liquid polyethylene glycols and mixtures thereof. A particular solvent is water.

Administration of compounds of the formula (I) or (Ia) may be in the form of a “prodrug”. A prodrug is an inactive form of a compound which is transformed in vivo to the active form. Suitable prodrugs include esters, phosphonate esters etc, of the active form of the compound.

It should be understood that in addition to the ingredients particularly mentioned above, the formulations may include other agents conventional in the art having regard to the type of formulation in question.

The compounds of the present invention may be suitable for the treatment of diseases in a human or animal patient. In one embodiment, the patient is a mammal including a human, horse, dog, cat, sheep, cow, or primate. In one embodiment the patient is a human. In a further embodiment, the patient is not a human.

As used herein, the term “effective amount” means that amount of a drug or pharmaceutical agent that will elicit the biological or medical response of a tissue, system, animal or human that is being sought, for instance, by a researcher or clinician. Furthermore, the term “therapeutically effective amount” means any amount which, as compared to a corresponding subject who has not received such amount, results in improved treatment, healing, prevention, or amelioration of a disease, disorder, or side effect, or a decrease in the rate of advancement of a disease or disorder. The term also includes within its scope amounts effective to enhance normal physiological function.

As used herein the term “treatment” refers to defending against or inhibiting a symptom, treating a symptom, delaying the appearance of a symptom, reducing the severity of the development of a symptom, and/or reducing the number or type of symptoms suffered by an individual, as compared to not administering a pharmaceutical composition comprising a compound of the invention. The term treatment encompasses the use in a palliative setting

Those skilled in the art will appreciate that in the preparation of the compounds of the invention it may be necessary and/or desirable to protect one or more sensitive groups in the molecule to prevent undesirable side reactions. Suitable protecting groups for use according to the present invention are well known to those skilled in the art and may be used in a conventional manner. See, for example, “Protective groups in organic synthesis” by T. W. Greene and P. G. M. Wuts (John Wiley & sons 1991) or “Protecting Groups” by PJ. Kocienski (Georg Thieme Verlag 1994). Examples of suitable amino protecting groups include acyl type protecting groups (e.g. formyl, trifluoroacetyl, acetyl), aromatic urethane type protecting groups (e.g. benzyloxycarbonyl (Cbz) and substituted Cbz), aliphatic urethane protecting groups (e.g. 9-fluorenylmethoxycarbonyl (Fmoc), t-butyloxycarbonyl (Boc), isopropyloxycarbonyl, cyclohexyloxycarbonyl) and alkyl type protecting groups (e.g. benzyl, trityl, chlorotrityl).

According to the first, second or eighteenth aspects of the invention, the cancers to be treated are leukemia, non-small cell lung cancer, colon cancer, CNS cancer, melanoma, ovarian cancer, renal cancer, prostate cancer or breast cancer. Most preferably, the cancer to be treated is leukemia or melanoma.

In a preferred embodiments of the first to ninth aspects, the compound according to formula (I) has the formula (Ia).

According to the tenth or nineteenth aspect of the invention, preferably the medicament is used to treat the following cancers; leukemia, non-small cell lung cancer, colon cancer, CNS cancer, melanoma, ovarian cancer, renal cancer, prostate cancer or breast cancer. Most preferably, the medicament is used to treat leukemia or melanoma.

In preferred embodiments of the tenth to fourteenth aspects, the compound according to formula (I) has the formula (Ia).

The antitumor effect of the compounds of the present invention may be applied as a sole therapy or may involve, in addition, one or more other substances and/or treatments. Such conjoint treatment may be achieved by way of the simultaneous, sequential or separate administration of the individual components of the treatment. In the field of medical oncology it is normal practice to use a combination of different forms of treatment to treat each patient with cancer such as a combination of surgery, radiotherapy and/or chemotherapy. In particular, it is known that irradiation or treatment with antiangiogenic and/or vascular permeability reducing agents can enhance the amount of hypoxic tissue within a tumour. Therefore the effectiveness of the compounds of the present invention may be improved by conjoint treatment with radiotherapy and/or with an antiangiogenic agent.

The individual components of such combinations can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms. The present invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment and the term “administering” is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other anti-neoplastic agents includes in principle any combination with any pharmaceutical composition useful for treating cancer.

When combined in the same formulation it will be appreciated that the two compounds must be stable and compatible with each other and the other components of the formulation and may be formulated for administration. When formulated separately they may be provided in any convenient formulation, conveniently in such a manner as are known for such compounds in the art.

Pharmaceutical compositions of the invention may be formulated for administration by any appropriate route, for example by the oral (including buccal or sublingual), rectal, nasal, topical (including buccal, sublingual or transdermal), vaginal or parenteral (including subcutaneous, intramuscular, intravenous or intradermal) route. Therefore, the pharmaceutical compositions of the invention may be formulated, for example, as tablets, capsules, powders, granules, lozenges, creams or liquid preparations, such as oral or sterile parenteral solutions or suspensions. Such pharmaceutical formulations may be prepared by any method known in the art of pharmacy, for example by bringing into association the active ingredient with the carrier(s) or excipient(s). Such pharmaceutical formulations may be prepared as enterically coated granules, tablets or capsules suitable for oral administration and delayed release formulations.

When a compound is used in combination with a second therapeutic agent active against the same disease, the dose of each compound may differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art.

In one embodiment of the invention according to the fifteenth aspect, there is provided a compound of formula (Ia) wherein R^(1a)-R^(1d), R², R³, R⁴ and A----B are as hereinbefore defined provided that:

-   -   (i) when R³ is H, then R^(1b) and R^(1d) are independently not         OH or OCH₂CH═C(CH₃)₂; and     -   (ii) when R³ is H, one of R^(1a-1d) is H and at least one of         R^(1a-1d) is CH₂CH═C(CH₃)₂, then A---B is not CH═CH.

According to the fifteenth or sixteenth aspects, in preferred embodiments of the invention two of R^(1a-1d) are H. In other preferred embodiments one of R^(1a-1d) is H, and in other embodiments none of R^(1a-1d) are H.

In a preferred embodiment, at least one of R^(1a-1d) is CH₂CH═C(CH₃)₂, and in another preferred embodiment at least one of R^(1a-1d) is OCH₂CH═C(CH₃)₂.

In certain embodiments at least one of R^(1a-1d) is hydroxyl, and in certain other embodiments at least two of R^(1a-1d) are hydroxyl. In another embodiment at least one of R^(1a-1d) is OR⁴ and R⁴ is methyl, and in yet another emboidment R⁴ is benzy.

In preferred embodiments of the invention according to the fifteenth or sixteenth aspects, at least one of R² or R³ is hydroxyl. In another preferred embodiment both R² and R³ are hydroxyl.

In another embodiment at least one of R² or R³ is OR⁴ and R⁴ is methyl, and in yet another embodiment R⁴ is benzyl.

In preferred embodiments of the invention, the group A----B in formula (Ia) or is CH═CH or CH═CHX—, where X═(CH₂)pCH₂, and p is an integer selected from the group consisting of 0, 1, 2 and 3.

In other embodiments of the invention the group A----B in formula (Ia) is CH₂—CH₂, or CH₂—CH₂X, where X═(CH₂)pCH₂, and p is independently an integer selected from the group consisting of 0, 1, 2 and 3.

In a preferred embodiment, the compound of formula (Ia) according to the fifteenth or sixteenth aspect has the formula (Ib):

-   -   Wherein, R⁴, R⁵ and R⁶ are each independently selected from the         group consisting of OMe, OEt,     -   OPr, O^(i)Pr, OBu, O^(i)Bu, O^(t)Bu, and OBn.

In another preferred embodiment the compound of formula (Ia) according to the fifteenth or sixteenth aspects has the formula (Ic) or (Id):

-   -   Wherein, R⁷, R⁸ and R⁹ are each independently selected from the         group consisting of OH, OMe, —OEt, OPr, O^(i)Pr, OBu, O^(i)Bu,         and O^(t)Bu.

In yet another preferred embodiment, the compound of formula (Ia) according to the fifteenth or sixteenth aspects has the formula (Ie) or (If)

-   -   Wherein, R⁷, R⁸ and R⁹ are each independently selected from the         group consisting of OH, OMe, OEt, OPr, O^(i)Pr, OBu, O^(i)Bu,         and O^(t)Bu.

Preferably, the compound according to the fifteenth or sixteenth aspects, or pharmaceutically acceptable salt or solvate thereof is selected from the group consisting of:

More preferably the compound according to the fifteenth or sixteenth aspects, or pharmaceutically acceptable salt or solvate thereof is selected from the group consisting of:

In one embodiment the compound according to the fifteenth or sixteenth aspects, or pharmaceutically acceptable salt or solvate thereof is selected from the group consisting of:

and

In another preferred embodiment the compound is USYDS18.

In another preferred embodiment, the compound according to the fifteenth aspect, or pharmaceutically acceptable salt or solvate thereof is selected from the group consisting of:

In preliminary in vitro assays to determine the anticancer activity of prenylated polyhydroxystilbene derivatives, the inventors observed structure dependent inhibition of cancerous cell growth for all derivatives, USYDS1 to USYDS7 and USYDS13. Most of these compounds, exhibited potent inhibition of almost all leukemia cell lines (CCRF-CEM, HL-60(TB), K-562, MOLT-4, RPMI-8226, SR). In some cell lines, growth was inhibited at nano-molar concentrations. USYDS1 displayed the most potent activity followed by USYDS10, USYDS9, USYDS2 then USYDS14 in the inhibition of cancerous cell growth. Structure dependent inhibition of cancerous cell growth for derivatives USYDS10 and 14 have also been observed. Structure-activity study shows the C-prenylation at the 2 position with the methoxy group at 3 position exhibits the highest potency towards inhibition of cancer cells.

Most of the pTHOS in this study have structures built on the piceatannol structure, a known naturally occurring compound, which displays a wide spectrum of biological activity. It is now attracting much attention on its anticancer properties because of its ability to inhibit proliferation of a wide variety of tumor cells, including leukemia, lymphoma; cancers of the breast, prostate, colon and melanoma. Its anticancer effects are suggested to mediate through cell-cycle arrest; upregulation of Bid, Bax, Bik, Bok, Fas; P21WAF1 down-regulation of Bcl-xL; BCL-2, cIAP, activation of caspases, loss of mitochondrial potential, and release of cytochrome c. Piceatannol has also been shown to inhibit the activation of some transcription factors, including NF-κB, which plays a central role in response to cellular stress caused by free radicals, ultraviolet irradiation, cytokines, or microbial antigens, JAK-1, a key transcription in the STAT pathway that controlling cellular activities in response to extracellular cytokines.

It has been observed that the pTHOS described in this study display a similar spectrum of biological activities to that exhibited by piceatannol. Furthermore, the pTHOS (ie. USYDS1, GI₅₀ values: 0.02 μM-5 μM) showed approximately 250 fold more potent than piceatannol (IC₅₀ values: 5 μM-100 μM) in inhibition of proliferation of almost all types of tumor cells. Therefore, the pTHOS present in this application will be an attractive lead for pharmaceutical research and development and as biological tools for further understanding the pathophysiology of cancer.

The potent cytotoxicity of compounds USYDS1 and compound USYDS2 may be explained in terms of their increased hydrophobicity, as demonstrated by their calculated Log partition coefficient (Log P) values. USYDS1 and USYDS2 have Log P values almost twice that of the hydroxystilbene resveratrol. The effects of Log P on therapeutic compounds relate primarily to tissue penetration and distribution. Higher Log P values will enable compounds to more easily cross cell membranes and enter cells.

In other preliminary experiments, particular examples of O- and C-prenylated hydroxystilbene derivatives were found to exhibit concentration dependent inhibition of the SIRT1 enzyme, a member of the Sirtuin family of proteins (silent information regulator two proteins, Sir 2).

Sirtuins have emerged as critical regulators for ageing and longevity in model organisms. Studies into genetics and physiology of sirtuins have shown that this enzyme family plays a role in a variety of cellular processes, including gene silencing, cell death, fatty acid metabolism, neuronal protection and life span extension.

Without being bound to any particular theory, it is proposed that modulation of SIRT1 activity could lead to the development of therapeutic agents for the treatment of diseases including cancer, metabolic syndrome, obesity, neurodegenerative disorder and aging-related diseases.

In a preferred embodiment, according to the fifteenth or sixteenth aspects the compound of formula (Ia) is chemically synthesised. In another preferred embodiment the compound of formula (Ia) is isolated from propolis, wherein the propolis originates from plants of the Lepidosperma genus. In yet another preferred embodiment, the compound of formula (Ia) is isolated from the resin, gum or exudate of the Lepidosperma genus.

According to the twentieth aspect of the invention, in the preparation of compounds of the formula (Ib), (Ic), (Id), (Ie) or (If), preferably the condensation reaction in step (ii) is carried out in the presence of a palladium catalyst. Preferably the palladium catalyst is palladium (II) acetate.

Preferably, the alkylation reaction in step (iii) is carried out in the presence of a metal hydride and a halogenated prenyl reagent. Preferably the metal hydride is sodium hydride; however, it would be appreciated by the person skilled in the art that other known metal hydrides can be used. Most preferably the halogenated prenyl reagent is BrCH₂CH═C(CH₃)₂.

In a preferred embodiment, the hydrogenation reaction in step (iv) or (vi) is carried out in the presence of a palladium catalyst in a mixture of solvents. Most preferably the palladium catalyst is palladium on carbon and the mixture of solvents comprises 1,4-cyclohexadiene and ethanol. However, it would be appreciated by the person skilled in the art that any other suitable reagents and solvents can be used.

In a preferred embodiment, the rearrangement reaction in step (v) is carried out in the presence of magnesium silicate particles (Florisil®), silica or alumina particles. In another preferred embodiment the rearrangement reaction can be carried out in the presence of microwave radiation or light. In yet another preferred embodiment, the rearrangement reaction in step (v) is carried out in the presence of magnesium silicate particles (Florisil®), silica or alumina particles and in the presence of microwave radiation or light.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a compilation of some of the isolated and synthesised prenylated polyhydroxystilbene derivatives.

FIG. 2 is a scheme summarising the synthesis of the novel O- and C-prenylated polyhydroxystilbenes derivatives.

FIG. 3 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS1.

FIG. 4 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS2.

FIG. 5 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS13.

FIG. 6 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS4.

FIG. 7 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS9.

FIG. 8 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS6.

FIG. 9 exhibits dose response curves for the inhibition of human cancerous cell growth, for various cell lines, by compound USYDS7.

FIG. 10. graphically represents the effect of stilbene compounds on free radical scavenging.

FIG. 11. graphically represents the concentration dependent inhibition of the NAD-dependent deacetylase sirtuin-2 (SIRT1) enzyme by stilbene compounds.

MODES FOR CARRYING OUT THE INVENTION

In order to better understand the nature of the invention a number of examples will now be described as follows:

Materials

Honey bees observed collecting from the sedges (Lepidosperma viscidum) were captured in plastic tubes, capped and frozen. Sections of the bee hind legs holding propolis were cut and pooled. Resin from plants were collected and stored at −20° C. until analysis. Plant samples were obtained and dried at 40° C. in a ventilated oven (Thermoline, NSW Australia) overnight to provide voucher specimens for identification by botanist, A/Prof Murray Henwood, John Ray Museum, University of Sydney, Sydney, Australia, and registered as Lepidosperma viscidum chemotype 1a, voucher number Duke 100222-42 and Lepidosperma viscidum chemotype 2a, voucher number Duke 100221-21.

76 Beehives made up of 3 10-frame boxes each fitted with a propolis mat under the hive cover lid were used to collect propolis samples. The individually numbered hives were used on location in the apiary sites situated in the central southern region of Kangaroo Island.

Thin layer chromatography sheets precoated with silica gel 60 F254 and silica gel 60H for normal-phase short-column chromatography (NPSCC) were purchased from Merck. TLC plates were visualized with a UVGL-58 mineral-light lamp, Multiband UV-2544/366.

All the chemicals used in the isolation and synthesis, including deuterated NMR solvents such as chloroform-d and methanol-d₄, deuterated dimethyl sulfoxide (d₆-dimethyl sulfoxide) were purchased from Sigma-Aldrich Pty Ltd (Castle Hill, NSW, Australia). Solvents including hexane, dichloromethane, ethyl acetate, isopropanol, ethanol, methanol, and acetic acid were of analytical grade and purchased from Ajax Fine Chem, Taren Point, NSW, Australia or Asia Pacific Specialty Chemical Ltd (APS).

Rotavapor model R-114 rotary evaporator with a water bath temperature ranging between 40-60° C. was used to evaporate the solvent fraction. Vacuum pump V-700 or Vacuubrand MD 4C NT diaphragm pump (Vacuubrand GMBH, Wertheim, Germany) with vacuum controller V-800 or V-850 is used. Final drying is carried out by a Napco 5831 vacuum oven (NAPCO, Salt Lake City, USA) using a DirectTorr vacuum pump (Sargent-Welch, Buffalo, USA).

Preparative HPLC was performed on a Shimadzu preparative gradient LC-8A system on a reversed-phase column (Grace, Alltima C18 5 μM 22 mm ID×250 mm), injection volume 500 μL, eluted with methanol (75%) and water at 10 mL/min and detected at 280 nm with a UV-Vis detector (Shimadzu SPD-20A). Analytical HPLC was performed on Shimadzu UFLC, LC-20AD pump, SIL-20A HT autosampler, with a Hewlett-Packard Column, NUCLEOSIL 100C18, 5 μm, 4 mm×125 mm, injection volume 20 μL, eluted with methanol-water-acetic acid (70:29.8:0.2) at 1 mL/min and detected at 230 nm with a UV-Vis detector (Shimadzu SPD-20A).

¹H and ¹³C Nuclear magnetic resonance (NMR) analyses were carried out on Varian 400 MHz System with a SMS autosampler (Palo Alto, Calif., USA). NMR spectra were referenced to tetramethylsilane (TMS). Mass spectra were obtained from a ThermoFinnigan TSQ 7000 (LC-MS/MS system) and a Finnigan Polaris Ion Trap MS/MS system (Finnigan, San Jose, USA) using an Xcalibur 1.2 data system.

Determination of the ¹H-NMR Chemical Profiles of the Plant and Propolis Specimens

Resin samples from the base of stem (0.1 g), bee hind leg (0.01 g) and beehive propolis (1.0 g) were extracted with ethyl acetate at room temperature for 15 min. The extracts were filtered, dried under reduced pressure and analyzed by ¹H-NMR and HPLC. Samples from the sedge type-1 were found to contain prenylated hydroxystilbenes and cinnamates as major constituents, whereas those from sedge type-2 showed only prenylated stilbenes as major constituents as discussed above. Propolis samples were subsequently selected for isolation of the components.

Isolation and Identification of Prenylated Polydroxystilbenes from Propolis of Kangaroo Island General Method

Propolis (10 g) was extracted with dichloromethane at room temperature with stirring for 1 hr. The extract was subjected to purification using normal-phase short column chromatography (NPSCC). A step-wise gradient of mobile phase (2×100 mL) consisting of dichloromethane (DCM) and ethyl acetate (EtOAc) at 0, 1, 2, 4, 8, 10, 15, 20, 50 and 100% was employed to elute the components which were analysed by TLC and NMR. Further purification of the compounds, if required, was subsequently carried out on the same NPSCC with different mobile phases consisting of either hexane and EtOAc or hexane and isopropanol. Normal-phase preparative HPLC was also employed when required to further purify the compounds (Shimadzu LC-8A). The compounds were eluted through a silica column (Altima® silica 10 μm, 10 cm×250 cm) at a flow rate of 10 mL/min with a mobile phase of 2% isopropanol in hexane at ambient temperature. The elution of compounds was monitored with a UV detector (UV/Vis SPD-20A) at 280 nm. Structures and identity of these purified compounds were characterized by ¹H- and ¹³C-NMR and mass spectrometry including high resolution mass spectrometry. Detailed structural analyses of the isolated compound were also carried out when needed by 2D-NMR using Gradient Heteronuclear Multiple Bond Coherence (GHMBC).

Isolated Prenylated Polyhydroxystilbene Derivatives (E)-2-(3-methyl-2-buten-1-yl)-4′,5-dihydroxy-3,3′-dimethoxystilbene (USYDS1)

Light yellow liquid. C₂₁H₂₄O₄ ¹H NMR (methanol-d₄, 400 MHz): R_(t) 14.64 min. δ 7.16 (d, J=16 Hz, H), 7.07 (d, J=4 Hz, H), 6.94 (dd, J=8, 4 Hz, H), 6.86 (d, J=16 Hz, H), 6.78 (d, J=8 Hz, H), 6.63 (d, J=4 Hz, H), 6.34 (d, J=4 Hz, H), 5.06 (m, H), 3.89 (s, 3H), 3.77 (s, 3H), 3.39 (m, 2H), 1.80 (bs, 3H), 1.66 (bs, 3H). ¹³C-NMR (CDCl₃, 100 MHz) δ 158.5, 154.4, 146.7, 145.6, 138.1, 130.6, 130.4, 130.3, 124.3, 123.7, 120.7, 120.6, 114.5, 108.2, 103.9, 98.2, 55.9, 55.7, 25.8, 24.5, 18.0; Cl-MS: m/z 339 (M−1)⁻, HRESIMS: m/z 341.1749 (M+H)⁺, calcd 341.1747 for C₂₁H₂₅O₄.

(E)-3-(3-methyl-2-butenyloxy)-4′,5-dihydroxy-3′-methoxystilbene (USYDS2)

Light yellow liquid. C₂₀H₂₂O₄ ¹H NMR (methanol-d₄, 400 MHz): R_(t) 15.46 min. δ 7.12 (d, J=4 Hz, 1H), 7.01 (d, J=16 Hz, 1H), 6.97 (dd, J=8, 4 Hz, 1H), 6.88 (d, J=16 Hz, 1H), 6.78 (d, J=8 Hz, 2H, 6.56 (m, 2H), 6.24 (m, 1H), 5.46 (m, 1H), 4.52 (d, J=4 Hz, 2H), 3.90 (s, 3H), 1.79 (bs, 3H), 1.76 (bs, 3H). ¹³C-NMR (CDCl₃, 100 MHz) δ 160.2, 156.9, 146.7, 145.5, 139.9, 138.6, 129.9, 129.2, 126.2, 120.6, 119.4, 114.7, 108.4, 105.9, 105.4, 101.5, 65.0, 55.9, 25.8, 18.2; Cl-MS m/z 325 (M−1)⁻, HRESIMS m/z 327.15938 (M+H)⁺, calcd 327.1591 for C₂₀H₂₃O₄.

(E)-4-(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxy-3′-methoxystilbene (USYDS13)

Light yellow liquid. C₂₀H₂₂O₄ ¹H NMR (methanol-d₄, 400 MHz): R_(t) 11.46 min. δ 7.08 (d, J=4 Hz, 1H), 6.93 (dd, J=8, 4 Hz, 1H), 6.91 (d, J=16 Hz, 1H), 6.78 (d, J=16 Hz, 1H), 6.77 (d, J=8 Hz, 1H), 6.46 (s, 2H), 5.23 (m, 1H), 3.89 (s, 3H), 3.28 (m, 2H), 1.76 (bs, 3H), 1.65 (bs, 3H); ¹³C NMR-GHMBC (methanol-d₄, 100 MHz): δ 157.4 (2C, C-3/5, H-2/6, H-1″), 149.3 (1C, C-3′, H-2′, H-5′, OCH₃), 147.6 (1C, C-4′, H-2′, H-6′), 137.7 (1C, C-1′, H-beta), 131.4 (1C, C-1, H-alpha), 131.3 (1C, C-3″, H-1″, H-4″, H-5″), 128.7 (1C, C-beta, H-2′, H-alpha), 127.6 (1C, C-alpha, H-2/6, H-6′), 124.8 (1C, C-2″, H-1″, H-4″, H-5″), 121.2 (1C, C-6′, H-2′, H-beta), 116.5 (1C, C-5′),116.1 (1C, C-4, H-2/6, H-1″), 110.4 (1C, C-2′, H-beta), 105.9 (2C, C-2/6, H-2/6, H-alpha), 56.5 (1C, OCH₃), 26.1 (1C, C-4″, H-5″), 23.5 (1C, C-1″), 18.1 (1C, C-5″, H-4″); Cl-MS: m/z 325 (M−1)⁻; HRMS: 325.14453 [M−1]⁻, (calculated 325.14398 for C₂₀H₂₁O₄).

(E)-3-(3-methyl-2-butenyloxy)-3′,4′,5-trihydroxystilbene (USYDS4)

Light yellow liquid. C₁₉H₂₀O₄ ¹H NMR (methanol-d₄, 400 MHz): R_(t) 18.62 min. δ 6.98 (d, J=4 Hz, 1H), 6.94 (d, J=16 Hz, 1H), 6.85 (dd, J=8, 4 Hz, 1H), 6.80 (d, J=16 Hz, 1H), 6.74 (d, J=8 Hz, 1H), 6.53 (m, 2H), 6.23 (m, 1H), 5.46 (m, 1H), 4.51 (d, J=4 Hz, 2H), 1.79 (bs, 3H), 1.76 (bs, 3H); ¹³C NMR (methanol-d₄, 100 MHz): δ 160.2, 158.2, 145.2, 145.1, 139.8, 136.9, 129.5, 128.5, 125.5, 119.9, 118.8, 114.9, 112.4, 105.2, 103.8, 100.7, 64.4, 24.4, 16.8; Cl-MS: m/z 311 (M−1)⁻; HREIMS: m/z 335.1252 (M+Na)⁺, calcd 335.1259 for C₁₉H₂₀O₄Na.

(E)-2,4-di(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxystilbene (USYDS6)

Off-white solid. C₂₄H₂₈O₃ ¹H NMR (methanol-d₄, 400 MHz): R_(t) 9.35 min. δ 7.30 (d, J=8 Hz, 2H), 7.11 (d, J=16 Hz, 1H), 6.80 (d, J=16 Hz, 1H), 6.76 (d, J=8 Hz, 2H), 6.63 (s, 1H), 5.21 (m, 1H), 5.10 (m, 1H), 3.41 (m, 2H), 3.35 (m, 2H), 1.81 (bs, 3H), 1.78 (bs, 3H), 1.68 (bs, 6H); Cl-MS: m/z 363 (M−1)⁻.

(E)-2,4-di(3-methyl-2-buten-1-yl)-3,3′,4′,5-tetrahydroxystilbene (USYDS7)

Off-white solid. C₂₄H₂₈O₄ ¹H NMR (methanol-d₄, 400 MHz): R_(t) 10.46 min. δ 7.07 (d, J=16 Hz, 1H), 6.94 (d, J=4 Hz, 1H), 6.79 (dd, J=8.4 Hz, 1H), 6.73 (d, J=16 Hz, 1H), 6.71 (d, J=8 Hz, 1H), 6.62 (s, 1H), 5.21 (m, 1H), 5.10 (m, 1H), 3.41 (m, 2H), 3.35 (m, 2H), 1.81 (bs, 3H), 1.78 (bs, 3H), 1.68 (bs, 6H); ¹³C NMR (methanol-d₄, 100 MHz): δ 153.23, 152.79, 145.04, 144.84, 134.98, 130.52, 130.26, 129.75, 128.67, 124.08, 123.94, 122.93, 118.56, 118.51, 115.32, 114.93, 112.30, 103.85, 24.54, 24.49, 24.46, 22.32, 16.74, 16.54; Cl-MS: m/z 379 (M−1)⁻¹; HRMS: 379.19148 [M−1]⁻, (calculated 379.19092 for C₂₄H₂₇O₄).

(E)-2-(3-methyl-2-buten-1-yl)-3,3′,4′,5-tetrahydroxystilbene (USYDS8)

C₁₉H₂₀O₄ ¹H NMR (acetone-d₆, 400 MHz): δ 7.07 (d, J=2 Hz, 1H), 7.15 (d, J=16 Hz, 1H), 6.89 (dd, J=8, 2 Hz, 1H), 6.81 (d, J=8 Hz, 1H), 6.80 (d, J=16 Hz, 1H), 6.63 (d, J=2 Hz, 1H), 6.35 (d, J=2 Hz, 1H), 5.15 (t, J=7 Hz, 1H), 3.42 (d, J=7 Hz, 2H), 1.81 (s, 3H), 1.65 (s, 3H). ¹³C NMR (acetone-d₆, 100 MHz): δ 6 155.9, 155.8, 145.4, 145.3, 138.4, 130.1, 129.7, 129.4, 124.5, 123.9, 117.4, 119.0, 115.4, 112.9, 103.4, 101.6, 24.0, 25.0, 17.2. HRESIMS: (m/z) 313.1434 (M+H)⁺, calcd 313.1440 for C₁₉H₂₁O₄.

(E)-2-(3-methyl-2-buten-1-yl)-3′,4′,5-trihydroxy-3-methoxystilbene (USYDS9)

C₂₀H₂₂O₄ ¹H NMR (acetone-d₆, 400 MHz): δ 7.16 (d, J=16 Hz, 1H), 7.07 (d, J=2 Hz, 1H), 6.90 (dd, J=8, 2 Hz, 1H), 6.85 (d, J=16 Hz, 1H), 6.82 (d, J=8 Hz, 1H), 6.71 (d, J=2 Hz, 1H), 6.39 (d, J=2.3 Hz, 1H), 5.09 (1H, t, J=7 Hz, 1H), 3.78 (s, 3H), 3.40 (d, J=7 Hz, 2H), 1.80 (s, 3H), 1.64 (s, 3H). ¹³C NMR (acetone-d₆, 100 MHz): δ 158.5, 156.3, 145.3 (2C), 138.0, 130.1, 130.0, 129.6, 123.7, 124.2, 119.1, 118.9, 115.3, 112.9, 103.7, 98.1, 55.0, 25.0, 23.9, 17.2. HRESIMS: m/z 327.1592 (M+H)⁺, calcd 327.1596 for C₂₀H₂₃O₄.

(E)-2-(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxy-3′-methoxystilbene (USYDS10)

C₂₀H₂₂O₄ ¹H NMR: (acetone-d₆, 400 MHz) δ 7.13 (d, J=16 Hz, 1H) 7.02 (dd, J=8, 2 Hz, 1H), 7.00 (s, 1H), 6.92 (d, J=8 Hz, 1H), 6.85 (d, J=16 Hz, 1H), 6.66 (d, J=2 Hz, 1H), 6.30 (d, J=2 Hz, 1H), 5.20 (t, J=7 Hz, 1H), 3.95 (s, 3H), 3.43 (d, J=7 Hz, 2H), 1.84 (s, 3H), 1.75 (s, 3H). ¹³C NMR (acetone-d₆, 100 MHz): δ 155.4, 154.4, 146.7, 145.7, 138.8, 133.6, 131.2, 130.1, 124.2, 122.5, 120.5, 117.7, 114.6, 108.4, 105.3, 102.5, 55.9, 25.8, 25.1, 18.0. HRESIMS: m/z 349.1411 (M+Na)⁺, calcd 349.1416 for C₂₀H₂₂O₄Na.

(E)-3-(3-methyl-2-butenyloxy)-4′,5-dihydroxystilbene (USYDS11)

C₁₉H₂₂O₃ ¹H NMR (CDCl₃, 400 MHz): δ 7.38 (dd, J=7, 2 Hz, 2H), 7.00 (d, J=16 Hz, 1H), 6.84 (d, J=16 Hz, 1H), 6.82 (dd, J=7, 2 Hz, 2H), 6.64 (t, J=2 Hz, 1H), 6.56 (t, J=2 Hz, 1H), 6.33 (t, J=2 Hz, 1H), 5.50 (t, J=7 Hz, 1H), 4.51 (d, J=7 Hz, 2H), 1.81 (s, 3H), 1.76 (s, 3H). ¹³C NMR (CDCl₃, 100 MHz): δ 160.4, 156.7, 155.4, 139.9, 138.4, 130.1, 128.8, 128.0 (2C), 126.3, 119.5, 115.6 (2C), 105.6, 105.5, 101.3, 64.9, 25.8, 18.2.

4-(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxydihydrostilbene (USYDS12)

C₁₉H₂₂O₄ ¹H NMR: (CD₃OD, 400 MHz) δ 6.97 (d, J=8 Hz, 2H), 6.66 (d, J=8 Hz, 2H), 6.13 (s, 2H), 5.22 (t, J=7 Hz, 1H), 3.24 (d, J=7 Hz, 2H), 2.72 (m, 2H), 2.64 (m, 2H), 1.74 (s, 3H), 1.64 (s, 3H). ¹³C NMR (CD₃OD, 100 MHz): δ 155.5 (2C), 154.9, 140.3, 132.9, 129.4, 128.9 (2C), 123.6, 114.6 (2C), 112.3, 106.5 (2C), 38.0, 36.8, 24.5, 21.7, 16.5.

(E)-2-(3-methyl-2-buten-1-yl)-3-(3-methyl-2-butenyloxy)-3′,4′,5-trihydroxystilbene (USYDS14)

Colourless solid, yield 12 mg. ESI-MS: m/z 379 [M−1]⁻, ¹H-NMR (methanol-d₄ 400 MHz): δ 7.07 (d, J=16 Hz, 1H), 6.96 (d, J=4 Hz, 1H), 6.80 (dd, J=8, 4 Hz, 1H), 6.79 (d, J=16 Hz, 1H), 6.74 (d, J=8 Hz, 1 H), 6.61 (d, J=4 Hz, 1H), 6.32 (d, J=4 Hz, 1H), 5.47 (m, 1H), 5.06 (m, 1H), 4.48 (m, 2H), 3.37 (m, 2H), 1.78 (m, 6H), 1.75 (m, 3H), 1.66 (m, 3H). ¹³C-NMR (100 MHz, CD₃OD): δ 16.75, 16.80, 23.84, 24.45, 24.54, 64.86, 99.04, 103.41, 112.39, 114.96, 118.71, 119.14, 120.14, 123.54, 123.97, 129.51, 129.70, 130.01, 136.78, 137.95, 145.08, 145.09, 155.59, 157.47. HRMS: 379.19148 [M−1]⁻, (calculated 379.19092 for C₂₄H₂₇O₄).

(E)-2,6-di(3-methyl-2-buten-1-yl)-3,3′,5,5′-tetrahydroxystilbene USYDS15

Colourless solid, yield 9 mg. ESI-MS: m/z 379 [M−1]⁻, ¹H NMR (methanol-d₄, 400 MHz): δ 6.91 (br t, 1H), 6.82 (d, J=16 Hz, 1H), 6.73 (d, J=1 Hz, 2H), 6.28 (s, 1H), 6.27 (d, J=16 Hz, 1H), 5.12 (m,2H), 3.26 (d, J=6 Hz 4H), 1.65 (m, 6H), 1.59 (m, 6H). ¹³C-NMR (100 MHz, CD₃OD): δ 16.75, 24.53, 25.60, 112.27, 114.86, 117.66, 118.24, 124.06, 124.63, 130.20, 133.33, 139.41, 144.73, 144.97, 153.04. HRMS: 379.19149 [M−1]⁻, (calculated 379.19092 for C₂₄H₂₇O₄).

(E)-2,6-di(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxy-3′-methoxystilbene USYDS18

Yield 5 mg. ESI-MS: m/z 393 [M−1]⁻. ¹H NMR: (CD₃OD, 400 MHz) δ 7.03 (d, J=2 Hz, 1H), 6.87 (d, J=17 Hz, 1H), 6.85 (dd, J=8, 2 Hz, 1H), 6.77 (d, J=8 Hz, 1H), 6.33 (d, J=17 Hz, 1H), 6.30 (s, 1H), 5.14 (br t, J=6 Hz, 2H), 3.88 (s, 3H), 3.26 (br d, J=6 Hz, 4H), 1.66 (br s, 6H), 1.60 (br s, 6H). ¹³C NMR (CD₃OD, 100 MHz): δ 18.37 (2C), 26.12 (2C), 27.20 (2C), 56.53, 102.58, 110.27, 116.42, 119.23 (2C), 121.03, 126.02 (2C), 126.31, 130.41 (2C), 131.7, 134.87, 140.95, 147.51, 149.26, 154.65 (2C). HRMS: 417.20363 [M+23]⁺, (calculated 417.20418 for C₂₅H₃₀O₄Na).

Chemical Modification and Synthesis of Prenylated Polyhydroxystilbenes A. Rearrangement of O-prenyl to C-prenyl Polyhydroxystilbene

A mixture of USYDS2 (5 mg) and Florisil (5 mg) in xylene (2 ml) was heated at 120° C. for 30 min in a microwave reactor (CEM Discover Microwave). The product was dried under reduced pressure and purified using NPSCC. Two rearrangement products were observed in a combined yield of approximately 30%.

The major product observed was (E)-4-(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxy-3′-methoxystilbene (USDYS13):

The minor product observed was (E)-2-(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxy-3′-methoxystilbene (USYDS10).

B. Synthesis of Prenylated Polyhydroxystilbenes 1. Preparation of 3,5-dihydroxybenzoic Acid Methyl Ester (1)

To a solution of 3,5-dihydroxybenzoic acid (10 g, 64.9 mmol) in anhydrous methanol (150 mL) acetyl chloride (2 mL, 28.1 mmol) was added dropwise. The mixture was heated at reflux under N₂ for 17 hours. The solvent was evaporated and the residue was dissolved in ethyl acetate (100 mL), then washed with saturated sodium hydrogen carbonate (3×100 mL). The combined organic layers were washed with water (2×100 mL), dried over sodium sulfate, filtered and concentrated in vacuo to afford 2 as a colourless solid (9.80 g, 90%): mp 167-168° C. (lit.¹ mp 168-169° C.); ¹H NMR (400 MHz, Acetone-d₆) δ 8.66 (s, 2H), 7.00 (d, J=2.4 Hz, 2H), 6.59 (t, J=2.4 Hz, 2H), 3.83 (s, 3H).

2. Preparation of 3-hydroxy-5-benzyloxybenzoic Acid Methyl Ester (2)

To a suspension of NaH (60% dispersion in mineral oil, 3.3 g, 137.3 mmol) in anhydrous N,N-dimethylformamide (DMF) (100 mL) was added a solution of 2 (10 g, 59.5 mmol) in anhydrous DMF (30 mL) at 0° C. under N₂, followed by the dropwise addition of benzyl bromide (6.4 mL, 53.8 mmol). The reaction mixture was stirred at room temperature for 2 hours, quenched with cold water (50 mL), acidified with cold 1 M HCl (20 mL) and extracted with diethyl ether (3×50 mL). The combined organic extracts were washed with water (2×50 mL), dried over sodium sulfate, filtered and evaporated in vacuo. The product was purified using NPSCC using a step-wise gradient consisting of chloroform/ethanol to afford 2 as an off-white solid (4.5 g, 60%)²: mp 97-98° C. (lit.³ mp 98° C.); C₁₅H₁₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 8.01 (s, 1H), 7.43-7.33 (m, 5H), 7.24 (dd, J=2.0, 1.2 Hz, 1H), 7.20 (dd, J=2.0, 1.2 Hz, 1H), 6.70 (t, J=2.0 Hz, 1H), 5.07 (s, 2H), 3.90 (s, 3H).

3. Preparation of 3-hydroxy-5-benzyloxybenzoic Acid (3)

To a solution of 2 (4.5 g, 16.7 mmol) in methanol (30 mL) was added 1 M NaOH (60 mL, 60 mmol). The resulting reaction mixture was stirred at 45° C. for 4 hours under N₂, followed by the addition of 1 M HCl (50 mL) to acidify the solution before extraction with ethyl acetate (3×40 mL). The combined organic extracts were washed with water (2×30 mL) and dried over sodium sulfate. The solvent was evaporated in vacuo to afford 3 as an off-white solid (4.02 g, 94%): mp 196-198° C.; C₁₄H₁₂O₄ ¹H NMR (400 MHz, Acetone-d₆) δ 7.42-7.33 (m, 5H), 7.21 (dd, J=2.4, 1.2 Hz, 1H), 7.11 (dd, J=2.4, 1.2 Hz, 1H), 6.68 (t, J=2.4 Hz, 1H), 5.07 (s, 2H).

4. Preparation of 3-hydroxy-5-methoxybenzoic Acid (4)

The title compound was prepared as described above by using methyl iodide instead of benzyl bromide to afford 4 as an off-white solid, mp 199-200° C. (lit.⁴ mp 199-200° C.); C₈H₈O₄ ¹H NMR (400 MHz, Acetone-d₆) δ 8.01 (s, 2H), 7.16 (t, J=1.2 Hz, 1H), 7.13 (t, J=1.2 Hz, 1H), 6.15 (t, J=2.4 Hz, 1H), 3.82 (s, 3H).

5. Preparation of 3-acetoxy-5-benzyloxybenzoic Acid (5)

To the solution of 3 (4.02 g, 17.6 mmol) in acetic anhydride (20 mL, 21.6 mmol) was added pyridine (10%, 0.15 mL, 1.76 mmol). The mixture was stirred at room temperature for 4 hours, quenched with water (30 mL), acidified by 0.1 M HCl (10 mL) and extracted with ethyl acetate (3×40 mL). The combined organic extracts were washed with water (2×30 mL), dried over sodium sulfate and filtered. The solvent was evaporated in vacuo and recrystallised from a mixture (1:1) of hexane/ethyl acetate to afford 5 as pinkish crystals (3.86 g, 96%)⁵: mp 133-134° C.; C₁₆H₁₄O₅ ¹H NMR (400 MHz, CDCl₃) δ 7.59 (dd, J=2.4, 1.2 Hz, 1H), 7.45 (dd, J=2.4, 1.2 Hz, 1H), 7.43-7.34 (m, 5H), 6.99 (t, J=2.4 Hz, 1H), 5.1 (s, 2H), 2.32 (s, 3H).

6. Preparation of 3-acetoxy-5-methoxybenzoic Acid (6)

The title compound was prepared as described in 5 was used to give 6 as an off white solid: mp 151-153° C.; C₁₀H₁₀O₅ ¹H NMR (400 MHz, CDCl₃) δ 7.50 (dd, J=2.0, 1.4 Hz, 1H), 7.43 (dd, J=2.0, 1.4 Hz, 1H), 6.90 (t, J=2.0 Hz, 1H), 3.86 (s, 3H), 2.32 (s, 3H).

7. Preparation of 3-methoxy-4-benzyloxybenzaldehyde (7)

To a suspension of NaH (60% dispersion in mineral oil, 1.6 g, 66.6 mmol) in anhydrous DMF (50 mL) at 0° C. under N₂ was added vanillin (4-hydroxy-3-methoxy benzaldehyde, 4 g, 26.3 mmol) in anhydrous DMF (20 mL) slowly via syringe, followed by the dropwise addition of benzyl bromide (3 mL, 25.2 mmol). The mixture was stirred at room temperature for 4 hours, quenched with cold water (30 mL), acidified with cold 1 M HCl (15 mL) and extracted with diethyl ether (3×40 mL). The combined organic extracts were washed with water (2×30 mL), dried over sodium sulfate and dried under reduced pressure to afford 7 as a colourless solid (6 g, 92%): mp 60-62° C. (lit.⁶ mp 61-62° C.); C₁₅H₁₄O₃ ¹H NMR (400 MHz, CDCl₃) δ 9.84 (s, 1H)), 7.43-7.31 (m, 7H)), 7.00 (d, J=8.4 Hz, 1H)), 5.25 (s, 2H)), 3.95 (s, 3H)).

8. Preparation of 4-methoxy-3-benzyloxybenzaldehyde (8)

The title compound was prepared as described in 7 to give 8 as a colourless solid: mp 61-63° C. (lit.⁷ mp 61-63° C.); C₁₅H₁₄O₃ ¹H NMR (400 MHz, CDCl₃) δ 7.49-7.31 (m, 7H)), 7.00 (d, J=8.1 Hz, 1H)), 5.20 (s, 2H)), 3.97 (s, 3H)).

9. Preparation of 4-ethenyl-2-methoxy-1-benzyloxybenzene (9)

To a suspension of methyltriphenylphosphonium bromide (6.5 g, 18.2 mmol) in anhydrous tetrahydrofuran (THF) (15 mL) under N₂ at 0° C. was added potassium tert-butoxide (2.3 g, 20.5 mmol) and the reaction mixture was warmed to room temperature. A solution of benzyl vanillin (7) (4 g, 16.5 mmol) in anhydrous THF (15 mL) was added dropwise and stirred for 2 hours, then quenched with cold water (20 mL), acidified with 0.1 M HCl (20 mL), and extracted with ethyl acetate (3×40 mL). The combined organic extracts were washed with 20% aq. sodium chloride (20 mL) then dried under reduced pressure. The product was purified using NPSCC using a step-wise mobile phase consisting of hexane/ethyl acetate to afford 9 as a colourless solid (3.37 g, 85%); mp 53-54° C. (lit.⁸ mp 50-51° C.); C₁₆H₁₆O₂ ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.28 (m, 5H), 6.99 (d, J=2 Hz, 1H), 6.90 (dd, J=4, 1 Hz, 1H), 6.83 (d, J=4 Hz, 1H), 6.68 (dd, J=17, 1 Hz, 1H), 5.64 (dd, J=17, 1 Hz, 1H), 5.17 (s, 2H), 5.14 (d, J=1 Hz, 1H), 3.92 (s, 3H).

10. Preparation of 5-ethenyl-2-methoxy-1-benzyloxybenzene (10)

The title compound was prepared as described in 9 to give 10 as an off-white solid; mp 68-69° C. (lit.⁹ mp 68-69° C.); C₁₆H₁₆O₂ ¹H NMR (400 MHz, CDCl₃): δ 7.48-7.31 (m, 5H), 7.01 (d, J=2 Hz, 1H), 6.97 (dd, J=8, 2 Hz, 1H), 6.85 (d, J=8 Hz, 1H), 6.64 (dd, J=17, 1 Hz, 1H), 5.56 (dd, J=17, 1 Hz, 1H), 5.17 (s, 2H), 5.12 (d, J=1 Hz, 1H), 3.92 (s, 3H).

11. Preparation of E-1-[3-acetoxy-5-benzyloxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (11)

To a solution of N,N-bis(2,6-diisopropyl) dihydro imidazolium chloride (0.093 g, 10%, 0.217 mmol) and Pd(OAc)₂ (0.048 g, 10%, 0.217 mmol) in xylene (3 mL) under N₂ at room temperature was added the acid chloride of 5 (0.7 g, 2.17 mmol) in xylene 2 mL, followed by the addition of 4-ethylmorpholine (0.04 mL, 0.316 mmol) and reagent 9 (0.627 g, 2.61 mmol) in xylene (3 mL). The mixture was heated at 130° C. for 18-22 hours. The solvent was evaporated in vacuo and the product was purified using NPSCC (hexane/ethyl acetate 3:1 as mobile phase) to give 11 as an yellow oil (0.33 g, 31.6%); C₃₁H₂₈O₅ ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.28 (m, 13H), 7.06 (d, J=2.0 Hz, 1H), 7.02 (d, J=17 Hz, 1H), 6.97 (dd, J=6, 2 Hz, 1H), 6.89 (m, 1H), 6.62 (t, J=2 Hz, 1H), 5.18 (s, 2H), 5.07 (s, 2H), 3.94 (s, 3H), 2.30 (s, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 169.39, 159.78, 151.81, 149.79, 148.32, 139.82, 137.00, 136.57, 130.48, 129.78, 128.63 (2C), 128.58 (2C), 128.10, 127.88, 127.55 (2C), 127.24 (2C), 126.02, 119.98, 113.95, 112.02, 110.50, 109.48, 107.28, 71.0, 70.27, 56.02, 21.19; Cl-MS m/z (%): 481 (M+1)⁺, 503 (M+Na)⁺; HRMS: m/z 503.1828 (M+Na)⁺, calcd 503.1834 for C₃₁H₂₈O₅Na.

12. Preparation of E-1-[3-acetoxy-5-benzyloxyphenyl]-2-[3-benzyloxy-4-methoxyphenyl]ethene (12)

The title compound was prepared as described in 11 to condense the acid chloride of 5 with reagent 10. The product was recrystallised from a mixture (2:1) of hexane/toluene to afford yellowish needle crystals; C₃₁H₂₈O₅ mp 133-134° C.; ¹H NMR (400 MHz, CDCl₃) δ 7.49-7.29 (m, 10H), 7.07 (d, J=2 Hz, 1H), 7.05 (dd, J=8, 2 Hz, 1H), 6.97 (d, J=16 Hz, 1H), 6.95 (t, J=2 Hz, 1H), 6.89 (d, J=8 Hz, 1H), 6.84 (t, J=2 Hz, 1H), 6.82 (d, J=16 Hz, 1H), 6.62 (t, J=2 Hz, 1H), 5.18 (s, 2H), 5.07 (s, 2H), 3.94 (s, 3H), 2.30 (s, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 169.39, 159.76, 151.80, 149.90, 148.34, 139.83, 137.05, 136.58, 129.94, 129.78, 128.64 (2C), 128.60 (2C), 128.11, 127.94, 127.58 (2C), 127.38 (2C), 125.88, 120.59, 112.00, 111.91, 111.81, 110.52, 107.22, 71.16, 70.27, 56.06, 21.18 (CH₃CO); Cl-MS m/z 481 (M+1)⁺, 503 (M+Na)⁺; FIRMS m/z 503.1832 (M+Na)⁺, calcd 503.1834 for C₃₁H₂₈O₅Na.

13. Preparation of E-1-[3-acetoxy-5-methoxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (13)

The title compound was prepared as described in 11 to condense the acid chloride of 6 with reagent 9 to give the title compound as an off white solid; mp 104-106° C.; C₂₅H₂₄O₅ ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.30 (m, 8H), 7.07 (d, J=2 Hz, 1H), 7.03 (d, J=16 Hz, 1H), 6.98 (dd, J=8, 2 Hz, 1H), 6.90 (d, J=16 Hz, 1H), 6.88 (m, 1H), 5.18 (s, 2H), 3.95 (s, 3H), 3.83 (s, 3H), 2.31 (s, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 169.41, 160.58, 151.83, 149.78, 148.31, 139.76, 137.00, 130.49, 129.72, 128.58 (2C), 127.88, 127.24 (2C), 126.07, 119.97, 113.94, 111.73, 109.67, 109.46, 106.54, 71.01, 56.02, 55.50, 21.18; Cl-MS m/z 405 [M+1]⁺; HRMS m/z 405.1695 (M+H)⁺, calcd 405.1702 for C₂₅H₂₅O₅.

14. Preparation of E-1-[3-hydroxy-5-benzyloxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (14)

To a solution of stilbene 11 (0.04 g, 0.083 mmol) in mixed solvent (MeOH / THF/H₂O, 3/3/3 mL) was added NaOH (0.02 g, 0.35 mmol) at 0° C. under N₂. The reaction mixture was warmed up to room temperature and stirred for 3 hours. The solution was acidified with 0.1 M HCl (5 mL) and extracted with ethyl acetate (3×20 mL). The combined organic extracts were washed with 20% aq. sodium chloride (10 mL), dried over sodium sulphate and evaporated in vacuo. The product was purified using NPSCC (mobile phase of hexane/ethyl acetate) followed by recrystallisation to afford 14 as a colourless solid (0.016 g, 44.5%); mp 114-115° C.; C₂₀H₂₆O₄ ¹H NMR NMR (400 MHz, Methanol-d₄) δ 7.45-7.28 (m, 10H), 7.06 (d, J=2 Hz, 1H), 7.00 (d, J=16 Hz, 1H), 6.98 (dd, J=8, 2 Hz, 1H), 6.87 (d, J=8 Hz, 1H), 6.86 (d, J=16 Hz, 1H), 6.71 (t, J=2.0 Hz, 1H), 6.59 (t, J=2 Hz, 1H), 6.38 (t, J=2 Hz, 1H), 5.18 (s, 2H), 5.07 (s, 2H), 3.95 (s, 3H); ¹³C NMR (100 MHz, Methanol-d₄) δ 160.29, 156.78, 149.76, 148.19, 139.92, 136.98, 136.84, 130.65, 129.20, 128.61 (2C), 128.57 (2C), 128.02, 127.88, 127.49 (2C), 127.26 (2C), 126.54, 119.94, 113.96, 109.45, 105.97, 105.69, 101.54, 71.02, 70.01, 56.03; Cl-MS: 461 (M+Na)⁺, 439 (M+1)⁺; HRMS m/z 439.1908 (M+H)⁺, calcd 439.1909 for C₂₉H₂₇O₄.

15. Preparation of E-1-[3-hydroxy-5-benzyloxyphenyl]-2-[3-benzyloxy-4-methoxyphenyl]ethene (15)

Basic hydrolysis of 12 was carried out as described for compound 14 to give the title compound as a colourless solid; mp 117-119° C.; C₂₉H₂₆O₄ ¹H NMR (400 MHz, Methanol-d₄) δ 7.49-7.30 (m, 10H), 7.07 (d, J=2.0 Hz, 1H), 7.06 (dd, J=8.4, 2.0 Hz, 1H), 6.96 (d, J=16.0 Hz, 1H), 6.87 (d, J=8.0 Hz, 1H), 6.79 (d, J=16.4 Hz, 1H), 6.99 (t, J=1.6 Hz, 1H), 6.57 (t, J=2.0 Hz, 1H), 6.38 (t, J=2.4 Hz, 1H), 5.19 (s, 2H), 5.07 (s, 2H), 3.90 (s, 3H). ¹³C NMR (100 MHz, Methanol-d₄) δ 160.20, 158.30, 149.68, 148.25, 139.70, 137.41, 137.31, 130.62, 128.16, 128.07 (2C), 128.05 (2C), 127.52, 127.41 (3C), 127.15 (2C), 126.62, 120.35, 111.95 (2C), 105.67, 104.09, 101.11, 70.88, 69.56, 55.10; Cl-MS: m/z 461 (M+Na)⁺, 439 (M+1)⁺; FIRMS m/z 439.1907 (M+H)⁺, calcd 439.1909 for C₂₉H₂₇O₄.

16. Preparation of E-1-[3-hydroxy-5-methoxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (16)

Basic hydrolysis of 13 was carried out as described for compound 14 to give the title compound as a yellowish solid; mp 110-112° C. C₂₃H₂₂O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.28 (m, 5H), 7.07 (d, J=2 Hz, 1H), 7.00 (d, J=16 Hz, 1H), 6.98 (dd, J=8, 2 Hz, 1H), 6.87 (d, J=17 Hz, 1H), 6.85 (d, J=8 Hz, 1H), 6.62 (t, J=2 Hz, 1H), 6.57 (t, J=2 Hz, 1H), 6.31 (t, J=2 Hz, 1H), 5.17 (s, 2H), 3.94 (s, 3H), 3.81 (s, 3H); ¹³C NMR (100MHz, CDCl₃) δ 161.08, 156.86, 149.73, 148.16, 139.85, 136.96, 130.69, 129.11, 128.57 (2C), 127.90, 127.29 (2C), 126.61, 119.95, 113.97, 109.47, 105.72, 104.73, 100.72, 71.03, 56.03, 55.36; Cl-MS, m/z 384 (M+Na)⁺, 363 (M+1)⁺; HRMS m/z 363.1592 (M+H)⁺, calcd 363.1596 for C₂₃H₂₃O₄.

17. Preparation of E-1-[3-(3-methyl-2-butenyloxy)-5-benzyloxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (17)

To the suspension of NaH (60% dispersion in mineral oil, 0.0051 g, 0.213 mmol) in anhydrous DMF (5 mL) under N₂ at 0° C., stilbene 14 (0.04 g, 0.091 mmol) was added dropwise in DMF (3 mL), followed by the dropwise addition of 3,3-dimetylallyl bromide (0.011 mL, 0.091 mmol).² The mixture was stirred at room temperature for 2-3 hours, then quenched with cold water (5 mL), acidified with cooled 0.1 M HCl (5 mL) and extracted with diethyl ether (3×10 mL). The combined organic extracts were washed with water (2×10 mL) and dried under reduced pressure. The product was purified using NPSCC (hexane/ethyl acetate as mobile phase) to give 17 as a yellowish oil (0.02 g, 43.3%); C₃₄H₃₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.28 (m, 10H), 7.07 (d, J=2 Hz, 1H), 7.01 (d, J=16 Hz, 1H), 6.96 (d, J=2 Hz, 1H), 6.90 (d, J=16 Hz, 1H), 6.87 (d, J=8 Hz, 1H), 6.73 (t, J=2 Hz, 1H), 6.68 (t, J=2 Hz, 1H), 6.48 (t, J=2 Hz, 1H), 5.52 (m, 1H), 5.17 (s, 2H), 5.07 (s, 2H), 4.52 (d, J=7 Hz, 2H), 3.95 (s, 3H), 1.80 (d, J=1 Hz, 3H), 1.76 (d, J=1 Hz, 3H); ¹³C NMR (100MHz, CDCl₃) δ 160.21, 160.12, 149.78, 148.14, 139.49, 138.35, 137.03, 136.94, 130.76, 128.94, 128.59 (2C), 128.56 (2C), 127.94, 127.86, 127.54 (2C), 127.24 (2C), 126.97, 119.87, 119.53, 113.98, 109.43, 105.38, 105.32, 101.14, 71.01, 70.09, 64.84, 56.03, 25.85, 18.22; Cl-MS: m/z 529 (M+Na)⁺, 507 (M+1)⁺; HRMS m/z 507.2528 (M+H)⁺, calcd 507.2535 for C₃₄H₃₅O₄.

18. Preparation of E-1-[3-(3-methyl-2-butenyloxy)-5-benzyloxyphenyl]-2-[3-benzyloxy-4-methoxyphenyl]ethene (18)

Prenylation of 15 was carried out as described for compound 17 to give the title compound as an off white solid; mp 88-90° C.; C₃₄H₃₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.49-7.32 (m, 10H), 7.08 (d, J=2 Hz, 1H), 7.06 (dd, J=8, 2 Hz, 1H), 6.97 (d, J=16 Hz, 1H), 6.89 (d, J=8 Hz, 1H), 6.82 (d, J=16 Hz, 1H), 6.71 (t, J=2 Hz, 1H), 6.66 (t, J=2 Hz, 1H), 6.47 (t, J=2 Hz, 1H), 5.53 (m, 1H), 5.20 (s, 2H), 5.07 (s, 2H), 4.52 (d, J=6 Hz, 2H), 3.91 (s, 3H), 1.80 (d, J=1 Hz, 3H), 1.75 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 161.04, 160.95, 150.51, 149.11, 140.24, 139.03, 137.81, 137.67, 130.91, 129.57, 129.27 (4C), 128.66, 128.58, 128.22 (2C), 128.05 (2C), 127.50, 121.10, 120.20, 112.48, 112.40, 105.95, 105.86, 101.60, 71.55, 70.47, 65.18, 56.36, 26.00, 18.33; Cl-MS: m/z 529 (M+Na)⁺, 507 (M+1)⁺; HRMS m/z 507.2530 (M+H)⁺, calcd 507.2535 for C₃₄H₃₅O₄.

19. Preparation of E-1-[3-(3-methyl-2-butenyloxy)-5-methoxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (19)

Prenylation of 16 was carried out as described for compound 17 to give the title compound as off white crystal; mp 66-69° C.; C₃₄H₃₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.45-7.28 (m, 5H), 7.08 (d, J=2 Hz, 1H), 7.02 (d, J=16 Hz, 1H), 6.98 (d, J=2 Hz, 1H), 6.90 (d, J=16 Hz, 1H), 6.87 (d, J=8 Hz, 1H), 6.67 (t, J=2 Hz, 1H), 6.64 (t, J=2 Hz, 1H), 6.40 (t, J=2 Hz, 1H), 5.53 (m, 1H), 5.17 (s, 2H), 4.53 (d, J=7 Hz, 2H), 3.96 (s, 3H), 3.81 (s, 3H), 1.81 (d, J=1 Hz, 3H), 1.76 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 160.91, 160.23, 149.76, 148.14, 139.46, 138.35, 137.04, 130.78, 128.90, 128.56 (2C), 127.87, 127.25 (2C), 127.02, 119.86, 119.56, 113.98, 109.44, 104.96, 104.49, 100.37, 71.02, 64.82, 56.04, 55.36, 25.86, 18.22; Cl-MS: m/z 453 (M+Na)⁺; HRMS m/z 453.2036 (M+Na)⁺, calcd 453.2042 for C₂₈H₃₀O₄Na.

20. Preparation of (E)-3-(3-methyl-2-butenyloxy)-4′,5-dihydroxy-3′-methoxystilbene (20, Equivalent to USYDS2)

To a solution of 17 (0.02 g, 0.035 mmol) in absolute ethanol (8 mL), was added 1,4-cyclohexadiene (3 mL, 0.030 mmol) and Pd-C (10%, 0.002 g). The mixture was stirred under N₂ and refluxed at 80° C. for 4 hours. The solution was filtered and dried under reduced pressure to give an oil residue which was purified by high performance liquid chromatography (HPLC) to afford 20 as a yellowish oil; C₂₀H₂₂O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.02 (d, J=2 Hz, 1H), 7.00 (d, J=16 Hz, 1H), 6.99 (d, J=2 Hz, 1H), 6.91 (dd, J=8, 1 Hz, 1H), 6.85 (d, J=16 Hz, 1H), 6.64 (t, J=2 Hz, 1H), 6.56 (t, J=2 Hz, 1H), 6.33 (t, J=2 Hz, 1H), 5.53 (m, 1H), 4.52 (d, J=7 Hz, 2H), 3.95 (s, 3H), 1.82 (d, J=1 Hz, 3H), 1.76 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 160.38, 156.70, 146.67, 145.69, 139.87, 138.37, 129.73, 129.29, 126.15, 120.63, 119.49, 114.54, 108.24, 105.58, 105.38, 101.29, 64.85, 55.92, 25.85, 18.22; Cl-MS: m/z 325 (M−1)⁻; HRMS m/z 327.1588 (M+H)⁺, calcd 327.1596 for C₂₀H₂₃O₄.

21. Preparation of 3-(3-methyl-2-butenyloxy)-4′,5-dihydroxy-3′-methoxydihydrostilbene (21)

The title compound was obtained by hydrogenation of the double bond on the bridging C═C during removal of the benzyl group of compound 20. The title compound was obtained as a light yellow oil; C₂₀H₂₄O₄ ¹H NMR 400 MHz, CDCl₃) δ 6.84 (d, J=8 Hz, 1H), 6.69 (dd, J=8, 2 Hz, 1H), 6.62 (d, J=2 Hz, 1H), 6.34 (t, J=2 Hz, 1H), 6.26 (t, J=2.0 Hz, 1H), 6.24 (t, J=2 Hz, 1H),), 5.46 (m, 1H), 5.46 (s, 1H), 4.68 (s, 1H), 4.53 (d, J=7 Hz, 2H), 3.84 (s, 3H), 2.85 (m, 4H), 1.80 (d, J=0.4 Hz, 3H), 1.73 (d, J=0.5 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 160.11, 156.44, 146.22, 144.46, 143.73, 138.23, 133.61, 120.95, 119.55, 114.15, 111.10, 107.89, 107.58, 99.62, 64.72, 55.84, 38.29, 37.23, 25.84, 18.18; Cl-MS: m/z 351 (M+Na)⁺; HRMS m/z 351.1566 (M+Na)⁺, calcd 351.1572 for C₂₀H₂₄O₄Na.

22. Preparation of (E)-3-(3-methyl-2-butenyloxy)-3′,5-dihydroxy-4′-methoxystilbene (22)

Removal of benzyl group of 18 was carried out as described for compound 20 to give the title compound as a light yellow oil; C₂₀H₂₂O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.13 (d, J=2 Hz, 1H), 6.98 (d, J=16 Hz, 1H), 6.97 (dd, J=8, 2 Hz, 1H), 6.86 (d, J=8 Hz, 1H), 6.86 (d, J=16 Hz, 1H), 6.64 (t, J=2 Hz, 1H), 6.57 (t, J=2 Hz, 1H), 6.33 (t, J=2 Hz, 1H), 5.60 (s, 1H), 5.52 (m, 1H), 4.77 (s, 1H), 4.52 (d, J=9 Hz, 2H), 3.91 (s, 3H), 1.82 (d, J=1 Hz, 3H), 1.76 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 160.37, 156.69, 146.51, 145.75, 139.83, 138.36, 130.87, 128.92, 126.78, 119.51, 119.40, 111.84, 110.62, 105.66, 105.48, 101.33, 65.18, 56.36, 26.00, 18.33; Cl-MS: m/z 349.1408 (M+Na)⁺, calcd 349.1416 for C₂₀H₂₂O₄Na.

23. Preparation of 3-(3-methyl-2-butenyloxy)-3′,5-dihydroxy-4′-methoxydihydro-stilbene (23)

The title compound was obtained by hydrogenation of double bond on the side chain during removal of the benzyl groups of compound 18. The title compound was obtained as a light yellow oil; C₂₀H₂₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 6.79 (d, J=2 Hz, 1H), 6.77 (d, J=4 Hz, 1H), 6.66 (dd, J=8, 2 Hz, 1H), 6.35 (t, J=2 Hz, 1H), 6.27 (m, 1H), 5.56 (s, 1H), 5.50 (m, 1H), 4.74 (s, 1H), 4.46 (d, J=7 Hz, 2H), 3.88 (s, 3H), 2.80 (m, 4H), 1.80 (d, J=1 Hz, 3H), 1.74 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 160.11, 156.42, 145.41, 144.80, 144.51, 138.18, 135.07, 119.73, 119.59, 114.59, 110.55, 107.81, 107.49, 99.64, 64.73, 55.00, 38.05, 36.89, 25.83, 18.18; Cl-MS: m/z 351 (M+Na)⁺; HRMS m/z 351.1566 (M+Na)⁺, calcd 351.1572 for C₂₀H₂₄O₃Na.

24. Preparation of E-1-[2-(3-methyl-2-butenyl)-5-hydroxy-3-benzyloxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (24)

To a solution of 17 (0.024 g, 0.061 mmol) in toluene (30 mL) was added 100-200 mesh Florisil (0.24 g, 10×) and heated at 110° C. under N₂, for 4 hours. The reaction mixture was filtered, evaporated in vacuo and the red-brown residue was purified using NPSCC (hexane/ethyl acetate as mobile phase) to afford a brownish solid (0.014 g, 58.3%). The product was recrystallised from hexane/ethyl acetate (3:1) mixture to give 24 as an off white solid; mp 145-150° C.; C₃₄H₃₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.28 (m, 10H), 7.20 (d, J=16 Hz, 1H), 7.06 (d, J=2 Hz, 1H), 6.97 (dd, J=8, 2 Hz, 1H), 6.88 (d, J=16 Hz, 1H), 6.87 (d, J=8 Hz, 1H), 6.68 (d, J=2 Hz, 1H), 6.40 (d, J=2 Hz, 1H), 5.18 (s, 2H), 5.17 (m, 1H), 5.05 (s, 2H), 4.69 (s, 1H), 3.94 (s, 3H), 3.48 (d, J=7 Hz, 2H), 1.73 (d, J=1 Hz, 3H), 1.67 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 157.60, 154.27, 149.76, 148.08, 138.31, 137.14, 137.04, 131.18, 130.59, 130.35, 128.56 (2C), 128.49 (2C), 127.86, 127.78, 127.23 (2C), 127.21 (2C), 124.74, 123.63, 121.13, 119.85,113.99, 109.47, 104.31, 99.53, 71.03, 70.29, 55.98, 25.77, 24.69, 18.01.

25. Preparation of E-1-[2-(3-methyl-2-butenyl)-5-hydroxy-3-methoxyphenyl]-2-[3-methoxy-4-benzyloxyphenyl]ethene (25)

Rearrangment of 19 was carried out as described for compound 24 to give a pinkish solid: mp 161-162° C.; C₂₈H₃₀O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.28 (m, 5H), 7.19 (d, J=16 Hz, 1H), 7.06 (d, J=2 Hz, 1H), 6.97 (dd, J=8, 2 Hz, 1H), 6.88 (d, J=16 Hz, 1H), 6.87 (d, J=8 Hz, 1H), 6.66 (t, J=2 Hz, 1H), 6.40 (t, J=2 Hz, 1H), 5.18 (s, 2H), 5.13 (m, 1H), 4.64 (s, 1H), 3.94 (s, 3H), 3.80 (s, 3H), 3.42 (d, J=6 Hz, 2H), 1.80 (d, J=1 Hz, 3H), 1.68 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 158.56, 154.35, 149.76, 148.07, 138.11, 137.04, 131.21, 130.63, 130.24, 128.56 (2C), 127.86, 127.22 (2C), 124.73, 123.61, 120.78, 119.84, 113.99, 109.42, 103.88, 98.21, 71.03, 55.97, 55.68, 25.77, 24.46, 17.98; Cl-MS: m/z 453 (M+Na)⁺, 431 (M+1)⁺; HRMS m/z 431.2217 (M+1)⁺, calcd 431.2222 for C₂₈H₃₁O₄.

26. Preparation of (E)-2-(3-methyl-2-buten-1-yl)-3,4′,5-trihydroxy-3′-methoxy-stilbene (26, Equivalent to USYDS10)

To a solution of 24 (0.02 g, 0.035 mmol) in absolute ethanol (6 mL) was added 1,4-cyclohexadiene (3 mL) and Pd—C (10%, 0.0035 mmol). The mixture was stirred under N₂ and heated at 80° C. for 4 hours. The solution was filtered and evaporated in vacuo to give an oil residue which was purified using NPSCC (hexane/ethyl acetate as mobile phase) followed by normal phase HPLC (2:1 hexane/isopropanol as mobile phase) to afford the title compound as a light yellow oil: Data analogous to that of USYDS10.

27. Preparation of (E)-2-(3-methyl-2-buten-1-yl)-5,4′-dihydroxy-3′,3-dimethoxy-stilbene (27, Equivalent to USYDS1)

The title compound was prepared using the procedure as described for compound 25 to give 27 as light yellow oil; C₂₁H₂₄O₄ ¹H NMR (400 MHz, CDCl₃) δ 7.17 (d, J=16 Hz, 1H), 7.01 (dd, J=12, 2 Hz, 1H), 7.0 (d, J=2 Hz, 1H), 6.91 (d, J=8 Hz, 1H), 6.87 (d, J=16 Hz, 1H), 6.66 (t, J=2 Hz, 1H), 6.36 (t, J=2 Hz, 1H), 5.15 (m, 1H), 4.64 (s, 1H), 3.94 (s, 3H), 3.80 (s, 3H), 3.42 (d, J=7 Hz, 2H), 1.81 (d, J=1 Hz, 3H), 1.68 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 158.57, 154.37, 146.68, 145.60, 138.18, 130.61, 130.44, 130.27, 124.28, 123.67, 120.73, 120.58, 114.55, 108.26, 103.88, 98.18, 55.88, 55.71, 25.79, 24.48, 17.98; Cl-MS: m/z 339 (M−1)⁻; HRMS m/z 363.1566 (M+Na)⁺, calcd 363.1572 for C₂₁H₂₄O₄Na.

28. Preparation of 2-(3-methyl-2-buten-1-yl)-5,4′-dihydroxy-3′,3-dimethoxydihydro-stilbene (28)

The title compound was obtained by hydrogenation of double bond on the side chain during removal of the benzyl group of compound 27. The title compound was obtained as a light yellow oil; C₂₁H₂₆O₄ ¹H NMR (400 MHz, CDCl₃) δ 6.86 (d, J=8 Hz, 1H), 6.70 (dd, J=8, 2 Hz, 1H), 6.64 (d, J=2 Hz, 1H), 6.30 (d, J=2 Hz, 1H), 6.24 (d, J=2 Hz, 1H), 5.48 (s, 1H), 5.07 (m, 1H), 4.67 (s, 1H), 3.85 (s, 3H), 3.78 (s, 3H), 3.28 (d, J=6 Hz, 2H), 2.82 (m, 4H), 1.74 (d, J=1 Hz, 3H), 1.66 (d, J=1 Hz, 3H); ¹³C NMR (100 MHz, CDCl₃) δ 158.63, 154.23, 146.23, 143.73, 142.01, 133.93, 130.59, 123.87, 120.92, 120.67, 114.21, 111.02, 107.96, 96.90, 55.84, 55.60, 37.19, 35.49, 25.76, 24.38, 17.95; Cl-MS: m/z 365 (M+Na)⁺; HRMS m/z 365.1723 (M+Na)⁺, calcd 365.1729 for C₂₁H₂₆O₄Na.

Biological Evaluations of Prenylated Polyhydroxystilbene Derivatives 1. Anticancer Activities of the Prenylated Polyhydroxystilbene Derivatives

A) Seven prenylated polyhydroxystilbene derivatives, namely USYDS1, USYDS2, USYDS4, USYDS6, USYDS7, USYDS9 and USYDS13 were evaluated for inhibition of cell growth, as shown in table 1 below, against the 60 cell lines at a range of concentrations (1×10⁻⁸-1×10⁻⁴ M) at the National Cancer Institute (NCI), USA.

Methodology of the In Vitro Cancer Screen: General Method Adopted from NCI

The human tumor cell lines were grown in RPMI 1640 medium containing 5% fetal bovine serum and 2 mM L-glutamine. For a typical experiment, cells were inoculated into 96 well microtiter plates in 100 μL at plating densities ranging from 5,000 to 40,000 cells/well depending on the doubling time of individual cell lines. After cell inoculation, the microtiter plates were incubated at 37° C., 5% CO₂, 95% air and 100% relative humidity for 24 h prior to addition of the drugs.

After 24 h, two plates of each cell line were fixed in situ with trichloroacetic acid (TCA), to represent a measurement of the cell population for each cell line at the time of drug addition. Experimental drugs are solubilized in dimethyl sulfoxide (DMSO) at 400-fold the desired final maximum test concentration and stored frozen prior to use. At the time of drug addition, an aliquot of frozen concentrate was thawed and diluted to twice the desired final maximum test concentration with complete medium containing 50 μg/mL gentamicin. Aliquots of 100 μL of the drug were added to the appropriate microtiter wells already containing 100 μL of medium, resulting in the required final drug concentrations.

Following drug addition, the plates were incubated for an additional 48 h at 37° C., 5% CO₂, 95% air, and 100% relative humidity. For adherent cells, the assay was terminated by the addition of cold TCA. Cells were fixed in situ by the gentle addition of 50 μL of cold 50% (w/v) TCA (final concentration, 10% TCA) and incubated for 60 minutes at 4° C. The supernatant was discarded, and the plates were washed five times with tap water and air dried. Sulforhodamine B (SRB) solution (100 μL) at 0.4% (w/v) in 1% acetic acid was added to each well, and plates were incubated for 10 minutes at room temperature. After staining, unbound dye was removed by washing five times with 1% acetic acid and the plates were air dried. Bound stain was subsequently solubilized with 10 mM trizma base, and the absorbance was read on an automated plate reader at a wavelength of 515 nm. For suspension cells, the methodology was the same except that the assay was terminated by fixing settled cells at the bottom of the wells by gently adding 50 μL of 80% TCA (final concentration, 16% TCA), and the absorbance was read on an automated plate reader at a wavelength of 515 nm. The GI₅₀ value (concentration required for 50% inhibition of cell growth), TGI value (concentration required for total inhibition of cell growth) and LC₅₀ value (concentration required for 50% cell lethality or death) were calculated for each of USYDS1, USYDS₂, USYDS4, USYDS6, USYDS7, USYDS9 and USYDS13, and the results are presented in tables 1 to 3 below.

TABLE 1 Effect of pPHOS USYDS1, USYDS2 and USYDS13 on human cancerous cells growth. Compounds USYDS1 (μM) USYDS2 (μM) USYDS13 (μM) GI₅₀ TGI LC₅₀ GI₅₀ TGI LC₅₀ GI₅₀ TGI LC₅₀ Leukemia CCRF-CEM 0.35 24.6 >100 5.06 >100 >100 6.56 >100 >100 HL-60 (TB) 0.02 0.06 68.9 1.34 9.77 >100 2.55 17.1 >100 K-562 0.04 11.7 >100 0.48 >100 >100 4.08 57.5 >100 MOLT-4 0.43 12.3 80.2 4.16 28.3 >100 2.95 13.7 >100 RPMI-8226 0.11 14.8 >100 4.32 52.8 >100 9.05 51.6 >100 SR 0.04 24.4 >100 0.54 >100 >100 2.80 >100 >100 Non-Small Cell Lung Cancer A549/ATCC 0.50 11.3 40.3 3.28 13.0 42.1 4.01 20.4 >100 EKVX 10.8 24.3 54.4 9.04 25.2 66.4 12.8 54.6 >100 HOP-62 0.44 16.0 42.2 4.44 18.5 43.4 5.66 20.4 53.7 HOP-92 0.06 12.3 39.6 3.98 21.1 60.1 5.96 30.5 >100 NCI-H226 3.13 13.3 45.6 5.43 19.1 46.4 10.9 50.1 >100 NCI-H23 0.28 15.1 62.5 2.89 23.6 >100 5.66 28.0 >100 NCI-H322M 11.8 25.7 55.9 9.64 22.7 52.3 5.90 28.5 >100 NCI-H460 0.23 1.38 42.0 2.47 10.9 58.7 3.09 15.6 >100 NCI-H522 0.02 — 44.8 1.14 3.57 14.7 2.13 59.8 25.6 Colon Cancer COLO 205 3.86 16.1 42.6 17.3 31.7 57.9 15.6 28.9 53.8 HCC-2998 1.07 11.5 35.8 4.61 17.3 46.4 11.0 30.6 85.3 HCT-116 0.30 11.1 40.4 2.09 11.9 40.0 4.33 15.6 45.6 HCT-15 0.05 16.1 >100 0.76 17.8 97.3 4.25 20.5 84.5 HT29 4.73 15.3 44.4 17.4 32.2 59.6 17.8 36.9 76.8 KM12 0.32 13.9 46.7 2.09 11.7 45.0 3.15 16.1 49.0 SW-620 0.04 15.1 44.3 0.53 14.0 40.7 3.98 15.9 42.8 CNS Cancer SF-268 0.25 14.1 50.2 4.50 19.2 51.6 6.17 32.9 >100 SF-295 0.65 16.2 55.7 3.94 14.7 49.2 5.22 23.5 77.5 SF-539 0.04 14.4 48.6 1.92 13.8 50.2 7.32 29.9 >100 SNB-19 0.17 19.3 51.5 5.02 20.4 53.6 8.02 24.2 64.5 SNB-75 0.03 20.2 72.0 2.14 10.3 55.8 5.47 36.6 >100 U251 0.37 10.9 36.0 2.96 14.7 41.0 5.47 20.7 57.6 Melanoma LOX IMVI 0.05 16.2 58.8 1.56 19.4 >100 4.48 20.2 80.0 MALME-3M 3.47 25.8 78.3 1.88 26.2 94.9 5.53 32.1 >100 M14 0.04 13.9 44.0 0.53 15.4 49.4 4.92 18.5 51.6 MDA-MB-435 0.03 13.8 43.0 0.34 11.0 41.3 3.08 15.9 >100 SK-MEL-2 0.32 10.6 38.0 1.25 9.59 41.6 3.09 9.37 40.2 SK-MEL-28 10.9 23.8 51.7 5.04 19.4 46.7 5.64 22.5 70.5 SK-MEL-5 0.05 0.75 5.99 1.58 11.5 34.3 2.50 8.54 29.2 UACC-257 2.53 17.7 45.4 7.89 20.6 46.0 5.35 18.1 44.0 UACC-62 1.62 18.3 46.6 3.73 15.9 44.6 5.30 19.7 58.8 Ovarian Cancer IGROV1 2.65 27.9 >100 5.84 46.7 >100 6.70 86.2 >100 OVCAR-3 0.26 — 37.3 3.02 10.7 36.1 7.98 39.8 >100 OVCAR-4 0.58 18.4 52.0 5.34 28.0 >100 9.68 74.8 >100 OVCAR-5 6.0 21.4 55.3 10.1 24.8 60.9 13.9 79.3 >100 OVCAR-8 0.08 11.7 50.0 2.80 14.8 44.6 5.48 59.8 >100 ADR-RES 0.03 — 70.7 0.40 4.95 >100 4.41 >100 >100 SK-OV-3 0.19 11.6 34.7 3.69 14.9 38.9 7.96 36.2 >100 Renal Cancer 786-0 0.48 14.0 >100 3.55 15.9 98.8 5.24 24.5 >100 A498 1.84 8.45 32.2 10.3 23.8 55.2 3.88 21.0 63.5 ACHN 0.07 14.0 44.6 3.76 17.5 52.1 5.11 >100 >100 CAKI-1 4.77 39.3 >100 6.13 >100 >100 5.90 >100 >100 RXF-393 0.03 — 39.1 1.35 5.46 23.6 5.48 18.9 46.5 SN12C 0.99 16.9 54.2 4.02 18.8 60.2 7.16 >100 >100 TK-10 4.10 15.7 40.5 4.69 14.3 40.1 6.98 31.4 >100 UO-31 0.25 16.0 51.3 2.60 16.9 48.9 4.16 38.1 >100 Prostate Cancer PC-3 0.42 16.1 47.9 3.84 18.7 65.8 6.36 >100 >100 DU-145 0.06 12.7 38.6 3.05 12.5 37.8 6.82 31.6 >100 Breast Cancer MCF7 0.23 13.0 52.9 1.24 15.0 41.0 0.68 19.9 82.7 MDA-MB-231 0.15 2.43 61.2 0.25 2.10 15.7 1.63 7.82 >100 BT-549 HS 578T 0.02 — >100 1.28 10.3 >100 2.40 15.0 >100 T-47D 0.72 19.8 67.4 5.66 33.6 >100 2.92 14.7 70.5 MDA-MB-468 0.36 8.37 33.2 2.72 9.41 36.8 1.68 7.63 >100

TABLE 2 Effect of pPHOS USYDS4, USYDS9 and USYDS6 on human cancerous cells growth Compounds USYDS4 (μM) USYDS9 (μM) USYDS6 (μM) GI₅₀ TGI LC₅₀ GI₅₀ TGI LC₅₀ GI₅₀ TGI LC₅₀ Leukemia CCRF-CEM 3.50 >100 >100 2.14 >100 >100 2.96 9.17 82.1 HL-60 (TB) 2.59 6.04 >100 0.20 0.43 0.95 2.07 4.83 15.4 K-562 2.35 >100 >100 0.33 13.9 >100 1.46 4.13 22.6 MOLT-4 4.31 21.6 >100 1.27 6.77 >100 1.59 4.27 16.8 RPMI-8226 2.35 25.4 >100 0.45 >100 >100 2.34 5.61 62.0 SR 0.95 >100 >100 — — — — — — Non-Small Cell Lung Cancer A549/ATCC 4.74 25.6 >100 2.62 41.5 >100 2.69 8.42 33.7 EKVX 9.55 59.6 >100 26.4 >100 >100 7.19 21.4 54.4 HOP-62 6.49 26.3 83.4 2.87 >100 >100 5.11 17.5 44.0 HOP-92 1.92 13.6 62.3 0.62 >100 >100 1.86 4.67 14.5 NCI-H226 5.14 23.1 78.0 18.6 57.9 >100 1.47 30.1 61.5 NCI-H23 3.42 20.8 81.9 1.43 >100 >100 3.38 15.7 47.6 NCI-H322M 12.8 54.7 >100 0.76 >100 >100 1.16 25.5 56.2 NCI-H460 3.65 20.3 >100 0.47 10.3 75.7 1.89 3.90 8.06 NCI-H522 1.07 2.54 6.04 0.15 0.45 44.8 2.18 5.15 17.8 Colon Cancer COLO 205 9.72 23.0 53.5 21.7 83.6 >100 16.0 30.2 57.2 HCC-2998 5.16 19.0 53.4 4.18 21.2 >100 2.81 8.78 32.2 HCT-116 4.55 16.0 40.6 1.40 12.8 75.0 1.89 3.78 7.54 HCT-15 1.77 34.9 >100 0.43 >100 >100 2.13 6.26 48.2 HT29 16.3 32.5 64.7 18.6 46.6 >100 5.44 17.6 48.3 KM12 3.27 14.2 46.5 0.52 12.2 63.9 2.21 4.37 8.64 SW-620 2.97 16.6 46.5 0.31 15.3 75.0 1.84 3.54 6.82 CNS Cancer SF-268 3.89 23.2 >100 0.63 37.6 >100 2.78 8.97 44.3 SF-295 7.29 24.3 70.9 2.60 20.5 >100 6.78 20.8 53.2 SF-539 3.45 22.3 97.5 0.28 1.02 >100 4.48 17.6 57.1 SNB-19 10.5 25.8 63.8 0.55 >100 >100 6.38 20.6 52.7 SNB-75 1.77 9.11 48.3 0.24 — >100 6.67 21.4 54.0 U251 3.67 15.6 45.5 0.59 19.6 >100 2.33 5.31 16.7 Melanoma LOX IMVI 3.82 19.3 58.5 0.64 58.6 >100 1.76 3.42 6.68 MALME-3M 7.25 30.6 >100 0.62 >100 >100 2.80 8.56 37.1 M14 0.91 15.4 46.3 0.30 5.69 >100 2.82 8.46 33.4 MDA-MB-435 0.36 21.6 >100 0.13 0.42 >100 3.37 10.8 42.1 SK-MEL-2 2.18 6.57 31.0 2.17 12.1 >100 13.1 33.6 86.1 SK-MEL-28 9.18 27.6 77.9 8.14 >100 >100 11.2 24.0 51.3 SK-MEL-5 1.42 8.35 30.0 0.39 2.52 >100 2.29 6.17 22.1 UACC-257 5.44 19.4 48.6 6.15 59.2 >100 3.49 14.4 39.8 UACC-62 4.53 16.8 44.4 3.22 34.8 >100 10.3 23.5 53.8 Ovarian Cancer IGROV1 3.74 44.8 >100 4.57 >100 >100 5.23 17.7 54.1 OVCAR-3 3.98 15.0 44.9 0.90 3.84 24.8 2.10 4.31 8.85 OVCAR-4 3.98 50.4 >100 5.48 >100 >100 4.40 16.1 43.5 OVCAR-5 15.9 51.4 >100 30.7 >100 >100 5.86 20.0 53.3 OVCAR-8 4.94 31.1 >100 0.50 38.3 >100 2.95 9.51 42.9 ADR-RES 1.11 48.3 >100 0.29 3.69 >100 3.46 15.2 51.2 SK-OV-3 8.91 57.0 >100 0.50 5.12 >100 13.9 27.1 53.2 Renal Cancer 786-0 4.41 20.5 >100 2.99 18.7 >100 2.46 6.96 28.8 A498 9.07 23.5 55.2 10.3 53.5 >100 11.7 25.0 53.1 ACHN 4.57 >100 >100 0.66 >100 >100 3.94 14.6 50.4 CAKI-1 5.25 >100 >100 19.1 >100 >100 8.37 22.6 55.7 RXF-393 2.17 15.3 46.0 0.23 0.63 >100 2.15 5.39 18.7 SN12C 3.94 20.0 74.1 1.76 83.5 >100 2.85 7.98 30.8 TK-10 3.87 9.66 >100 5.34 41.2 >100 3.36 9.33 31.2 UO-31 3.31 20.7 66.4 0.46 >100 >100 2.51 7.50 33.5 Prostate Cancer PC-3 4.47 22.0 87.9 2.92 >100 >100 1.80 3.89 8.44 DU-145 6.37 44.6 >100 0.35 2.62 >100 3.47 12.9 40.1 Breast Cancer MCF7 2.57 18.2 51.1 2.81 >100 >100 4.02 18.0 60.5 MDA-MB-231 1.04 18.4 >100 0.38 2.61 >100 1.72 4.54 17.2 HS 578T 1.00 7.11 >100 0.20 0.73 >100 1.67 5.29 35.8 T-47D 2.13 6.93 >100 4.78 36.9 >100 5.03 20.3 57.9 MDA-MB-468 2.53 9.51 55.1 2.42 8.48 >100 2.30 6.39 26.0

TABLE 3 Effect of pPHOS USYDS7 on human cancerous cells growth Compound USYDS7 (μM) GI₅₀ TGI LC₅₀ Leukemia CCRF-CEM 2.73 8.48 71.2 HL-60 (TB) 2.39 5.06 13.8 K-562 2.14 4.78 17.4 MOLT-4 2.01 5.19 26.5 RPMI-8226 2.31 6.01 61.1 Non-Small Cell Lung Cancer A549/ATCC 1.88 4.06 8.78 EKVX 4.25 14.7 44.9 HOP-62 3.67 15.0 51.6 HOP-92 1.52 4.21 14.2 NCI-H226 2.28 6.24 28.1 NCI-H23 1.81 5.22 29.5 NCI-H322M 5.29 18.1 44.7 NCI-H460 1.64 3.22 6.33 NCI-H522 1.61 3.57 7.92 Colon Cancer COLO 205 1.79 4.34 12.2 HCC-2998 1.95 4.26 9.34 HCT-116 1.57 3.02 5.82 HCT-15 1.29 3.78 22.0 HT29 3.64 10.8 45.8 KM12 1.93 3.88 7.79 SW-620 1.58 3.19 6.42 CNS Cancer SF-268 2.26 5.47 22.7 SF-295 3.37 11.4 44.6 SF-539 2.21 6.14 35.2 SNB-19 2.80 8.64 39.9 SNB-75 2.98 12.8 49.1 U251 1.49 2.93 5.74 Prostate Cancer PC-3 2.02 4.57 11.3 DU-145 2.65 7.62 28.3 Melanoma LOX IMVI 1.71 3.41 6.81 MALME-3M 1.97 4.46 10.4 M14 1.79 3.71 7.73 MDA-MB-435 2.29 6.47 31.7 SK-MEL-2 3.20 7.82 42.9 SK-MEL-28 3.65 11.8 36.7 SK-MEL-5 1.80 3.68 7.54 UACC-257 2.18 5.93 23.0 UACC-62 2.80 7.84 31.5 Ovarian Cancer IGROV1 3.12 8.39 36.7 OVCAR-3 1.82 3.36 6.19 OVCAR-4 2.85 11.1 66.8 OVCAR-5 3.04 11.6 39.0 OVCAR-8 1.68 3.65 7.95 ADR-RES 2.23 7.90 42.4 SK-OV-3 5.98 19.4 46.5 Renal Cancer 786-0 1.99 4.47 10.2 A498 11.1 23.8 50.8 ACHN 2.58 9.12 37.6 CAKI-1 4.24 16.2 49.0 RXF-393 1.53 3.23 6.80 SN12C 1.65 3.16 6.03 TK-10 3.62 11.2 34.3 UO-31 1.60 3.46 7.46 Breast Cancer MCF7 2.10 12.0 57.9 MDA-MB-231 1.49 3.89 10.7 HS 578T 1.84 8.09 50.9 T-47D 1.88 6.09 43.5 MDA-MB-468 2.12 4.84 13.9

In summary, all the prenylated polyhydroxystilbene derivatives USYDS1 to USYDS9 and USYDS13 exhibited structure dependent inhibition of cancerous cell growth. In some cell lines, growth was inhibited at nano-molar concentrations. USYDS1 displayed the most potent activity followed by USYDS9 then USYDS2 in the inhibition of cancerous cell growth. The other pPHOS compounds tested were shown to be moderate inhibitors. FIGS. 3 to 9 show dose response curves for the inhibition of human cancerous cell growth, for the various cell lines exhibited in the table above, by compounds USYDS1 to USYDS9 and USYDS13.

Worthy of note is that these pPHOS required at least a 10 fold excess in concentration to cause cell death (LC₅₀ values) or cause necrosis, over that required to inhibit cell growth (GI₅₀ values). This indicates that the pPHOS were likely to cause the cancer cells to undergo programmed cell death (apoptosis) or cell cycle arrest.

B) Two prenylated polyhydroxystilbene derivatives, namely USYDS10 and USYDS14 were evaluated for inhibition of cell growth, as shown in Table 4A and 4B below, against the cell lines indicated at a range of concentrations (1×10⁻⁸-1×10⁻⁴ M) at the National Cancer Institute (NCI), USA.

TABLE 4A Inhibitory effect on cancer cells growth of USYDS10 GI50 TGI LC50 Leukemia HL-60(TB) 3.27E−7 1.88E−6 >1.00E−4  K-562 3.86E−7 3.17E−5 >1.00E−4  MOLT 5.96E−7 1.99E−5 >1.00E−4  RPMI 3.24E−7 1.63E−5 >1.00E−4  SR 1.88E−7 >1.00E−4  >1.00E−4  Non-Small Cell Lung Cancer A549/ATCC 6.60E−7 2.25E−5 >1.00E−4  EKVX 1.22E−5 7.34E−5 >1.00E−4  HOP 5.81E−7 1.77E−5 4.23E−5 HOP 3.98E−7 2.63E−5 9.32E−5 NCI-H226 5.35E−6 3.50E−5 >1.00E−4  NCI-H23 3.80E−7 1.52E−5 7.83E−5 NCI-H322M 1.18E−5 3.14E−5 8.37E−5 NCI-H460 3.81E−7 1.29E−5 6.61E−5 NCI-H522 3.49E−7 1.46E−5 4.22E−5 Colon Cancer COLO 1.53E−5 2.97E−5 5.77E−5 HCC-2998 1.81E−6 1.19E−5 3.98E−5 HCT-116 4.80E−7 1.30E−5 4.27E−5 HCT-15 4.98E−7 2.09E−5 >1.00E−4  HT29 5.72E−6 2.13E−5 6.90E−5 KM12 4.97E−7 1.53E−5 8.58E−5 SW-620 3.62E−7 2.15E−5 8.55E−5 CNS Cancer SF-268 1.40E−6 4.96E−5 >1.00E−4  SF-295 2.01E−6 1.91E−5 >1.00E−4  SF-539 3.76E−7 1.29E−5 4.17E−5 SNB-19 6.13E−7 2.28E−5 >1.00E−4  SNB-75 2.36E−7 1.22E−5 3.61E−5 U251 4.19E−7 1.62E−5 7.88E−5 Melanoma LOX IMVI 5.72E−7 1.93E−5 8.00E−5 MALME-3M 1.87E−5 4.17E−5 9.29E−5 M14 2.84E−7 1.42E−5 4.16E−5 MDA-MB-435 3.85E−8 2.56E−7 >1.00E−4  SK-MEL-2 5.76E−7 3.26E−5 >1.00E−4  SK-MEL-28 5.72E−7 1.69E−5 4.30E−5 SK-MEL-5 2.91E−7 1.27E−5 3.60E−5 UACC-257 1.17E−5 2.94E−5 7.37E−5 UACC-62 5.21E−7 1.55E−5 4.28E−5 Ovarian Cancer IGROV1 2.03E−6 >1.00E−4  >1.00E−4  OVCAR-3 3.72E−7 2.03E−5 >1.00E−4  OVCAR-4 1.03E−6 1.82E−5 5.77E−5 OVCAR-5 8.92E−6 2.80E−5 8.31E−5 OVCAR-8 4.67E−7 6.50E−5 >1.00E−4  NCI/ADR-RES 1.83E−7 8.04E−7 >1.00E−4  SK-OV-3 4.22E−7 1.14E−5 3.42E−5 Renal Cancer 786-0 6.81E−7 2.01E−5 7.45E−5 A498 2.17E−6 7.85E−6 3.48E−5 ACHN 9.03E−7 3.79E−5 >1.00E−4  CAKI-1 5.39E−7 6.68E−5 >1.00E−4  RXF 393 2.21E−7 3.76E−5 SN12C 6.71E−7 2.98E−5 >1.00E−4  TK-10 9.55E−6 5.15E−5 >1.00E−4  UO-31 8.78E−7 2.17E−5 9.52E−5 Prostate Cancer PC-3 2.27E−6 3.12E−5 >1.00E−4  DU-145 4.42E−7 2.05E−5 >1.00E−4  Breast Cancer MCF7 3.32E−7 1.30E−5 5.06E−5 MDA-MB-231/ATCC 6.42E−7 3.20E−5 >1.00E−4  HS 578T 2.92E−7 2.60E−5 >1.00E−4  BT-549 7.98E−7 1.90E−5 4.93E−5 T-47D 1.47E−6 2.47E−5 >1.00E−4  MDA-MB-468 3.73E−7 1.44E−5 4.49E−5

TABLE 4B Inhibitory effect on cancer cells growth of USYDS14 GI50 TGI LC50 Leukemia HL-60(TB) 4.06E−6 1.63E−5 >1.00E−4  K-562 8.33E−7 1.42E−5 >1.00E−4  MOLT-4 4.03E−6 2.10E−5 >1.00E−4  RPMI-8226 4.07E−6 2.45E−5 >1.00E−4  SR 6.70E−7 2.46E−5 >1.00E−4  Non-Small Cell Lung Cancer A549/ATCC 4.33E−6 1.65E−5 4.55E−5 EKVX 6.45E−6 2.12E−5 5.17E−5 HOP-62 1.43E−6 3.77E−6 9.98E−6 HOP-92 1.49E−6 7.58E−6 3.29E−5 NCI-H226 1.72E−6 3.60E−6 7.52E−6 NCI-H23 2.92E−6 1.73E−5 5.76E−5 NCI-H322M 1.25E−5 2.57E−5 5.28E−5 NCI-H460 3.87E−6 1.49E−5 5.12E−5 NCI-H522 7.50E−7 2.26E−6 5.85E−6 Colon Cancer COLO 205 1.46E−5 2.85E−5 5.57E−5 HCC-2998 2.90E−6 1.06E−5 4.07E−5 HCT-116 4.20E−6 1.60E−5 4.66E−5 HCT-15 2.06E−6 1.04E−5 4.33E−5 HT29 1.40E−5 2.91E−5 6.09E−5 KM12 3.74E−6 1.44E−5 5.74E−5 SW-620 2.88E−6 1.67E−5 6.38E−5 CNS Cancer SF-268 4.65E−6 1.78E−5 4.85E−5 SF-295 3.91E−6 1.53E−5 4.31E−5 SF-539 2.96E−6 1.05E−5 3.78E−5 SNB-19 5.01E−6 2.01E−5 5.22E−5 SNB-75 1.79E−6 7.80E−6 2.93E−5 U251 3.09E−6 1.27E−5 3.94E−5 Melanoma LOX IMVI 1.36E−6 3.37E−6 8.35E−6 MALME-3M 3.14E−6 2.18E−5 5.48E−5 M14 2.95E−6 1.36E−5 5.00E−5 MDA-MB-435 3.73E−7 8.45E−6 4.00E−5 SK-MEL-2 3.72E−6 1.25E−5 4.12E−5 SK-MEL-28 3.05E−6 1.64E−5 4.16E−5 SK-MEL-5 5.54E−7 1.87E−6 4.46E−6 UACC-257 1.02E−5 2.35E−5 5.40E−5 UACC-62 2.61E−6 1.32E−5 3.91E−5 Ovarian Cancer IGROV1 3.65E−6 1.37E−5 6.54E−5 OVCAR-3 3.52E−6 1.35E−5 4.11E−5 OVCAR-4 2.98E−6 1.36E−5 3.86E−5 OVCAR-5 1.24E−5 2.58E−5 5.36E−5 OVCAR-8 3.59E−6 1.56E−5 6.49E−5 NCI/ADR-RES 7.80E−7 1.14E−5 4.93E−5 SK-OV-3 2.85E−6 1.37E−5 3.72E−5 Renal Cancer 786-0 3.44E−6 1.18E−5 4.00E−5 A498 1.30E−5 2.66E−5 5.46E−5 ACHN 2.98E−6 1.25E−5 3.65E−5 CAKI-1 3.59E−6 1.75E−5 4.54E−5 RXF 393 1.07E−6 3.58E−6 1.48E−5 SN12C 3.61E−6 1.51E−5 4.20E−5 TK-10 3.79E−6 1.21E−5 3.54E−5 UO-31 3.71E−6 1.79E−5 4.63E−5 Prostate Cancer PC-3 6.65E−6 2.16E−5 5.50E−5 DU-145 8.20E−6 2.11E−5 4.75E−5 Breast Cancer MCF7 2.74E−6 1.18E−5 4.19E−5 MDA-MB-231/ATCC 3.15E−6 1.43E−5 4.53E−5 HS 578T 2.71E−6 2.05E−5 >1.00E−4  BT-549 4.27E−6 1.63E−5 4.69E−5 T-47D 2.71E−6 7.84E−6 6.18E−5 MDA-MB-468 1.42E−6 4.12E−6 1.64E−5

In summary, all the prenylated polyhydroxystilbene derivatives USYDS10 and USYDS14 exhibited structure dependent inhibition of cancerous cell growth.

2. Calculated Log Partition Coefficient (Log P) Values of Various Hydroxystilbenes

The calculated Log P values for USYDS1 and USYDS2 and known hydroxystilbenes are presented in the table 5 below.

TABLE 5 Calculated Log P values of various hydroxystilbenes Compound USYDS2 5.37 Rhapontigenin 2.82 Compound USYDS1 5.58 Compound USYDS1 without 3.49 a prenyl group. Resveratrol 3.14 Pinosylvin 3.68 Piceatannol 1.90

The potent inhibition of pPHOS compounds, for example USYDS1 and USYDS₂, may be explained in terms of their increased hydrophobicity, as demonstrated by their calculated Log partition coefficient (Log P) values. USYDS1 and USYDS2 have Log P values almost twice that of the hydroxystilbene resveratrol. The effects of Log P on therapeutic compounds relate primarily to tissue penetration and distribution. Higher Log P values will enable compounds to more easily cross cell membranes and enter cells.

3. Effect of Prenylated Polyhydroxystilbene Derivatives on UV-Irradiated Human Skin Cells

Normal adult human keratinocytes (NHK) cells (Invitrogen, Vic, Australia) were cultured in keratinocyte growth medium Epilife supplemented with calcium and human keratinocyte growth supplement (HKGS, containing 0.2 ng EGF per mL, 5 mg insulin per mL, 5 mg transferrin per mL, 0.18 mg hydrocortisone per mL, and 0.2% bovine pituitary extract) (Invitrogen, Vic, Australia) in 12-well culture plates until the subconfluent state is reached. Cells were cultured to a density of 5×10³ cells per mL/well in a 24-well plate for 24 h at 37° C. in a humidified incubator with 5% carbon dioxide and tested according to the protocols described below:

Determination of Optimal Doses of UV Irradiation

Cells, seeded at a density as described above, were washed twice with PBS (phosphate buffered saline) then irradiated with a range of UVA and UVB doses known as MED (minimal erythema dose, 1 MED=25.43/light intensity). Cells were replaced with growth medium and incubated for about 24 h at 37° C. in a humidified incubator with 5% carbon dioxide. Cell viability was measured using the MTS assay (CellTiter 96® AQ_(ueous) One Solution Cell Proliferation Assay) (Promega, Vic Australia).

Rescue Assay

Cells were washed twice with PBS, replaced with a thin layer of PBS, then irradiated with the optimal doses of UVA and UVB as determined above. Immediately after irradiation, cells were replaced with fresh culture medium containing the test samples at a range of concentrations, and further incubated in a humidified CO₂ incubator at 37° C. for 24 hr. Supernatants were collected and kept at −80° C. until determination of PGE2 and cytokines (IL1, 6, 8, 10 & 12) concentration using ELISA kits.

Protective Assay

Cells were washed twice with PBS, replaced with a thin layer of PBS containing different concentrations of the test compounds then irradiated with optimal doses of UVA and UVB as determined above. Immediately after irradiation, cells were replaced with fresh culture medium and further incubated in a humidified CO₂ incubator at 37° C. for 24 h. Supernatants were be collected and kept at −80° C. until determination of PGE2 and cytokines (IL1, 6, 8, 10 & 12) concentration using ELISA kits.

Sham-treated control cultures were handled identically but not exposed to UV irradiation. Stilbenes compounds including USYDS1, 2, 13, 5, and 7 and propolis extract were tested at 0.1, 1 and 10 μM or μg/mL.

Results for the determination of optimal doses of UV irradiation revealed that at 1 MED of UVA and UVB irradiation, there is no significant effect on cell viability. Thus, this condition was chosen for investigation of the effects of stilbenes and propolis extract on levels of cytokines in the rescue assay.

In preliminary investigation on modulation of cytokine productions in UV irradiated human epidermal keratinocytes (HEK) it was observed that a mixture of USYDS1 and USYDS2 moderately inhibit the production of IL-6, TGFα, G-CSF and GM-CSF (2-3 fold). However, it was found that the prenylated polyhydroxystilbene derivatives significantly increased IL-8 and IL-lra production (4-5 fold) from UV irradiated cells. IL-8 is known to play a role in the onset of immunity response. IL-1rα (naturally occurring cytokine receptor antagonist) on the other hand plays an important role in inhibition of deleterious effect of IL-1 during inflammatory processes. Therefore, these preliminary results demonstrate that the prenylated polyhydroxystilbene derivates of the present invention may be good candidates for the treatment of conditions associated with immunity suppression and inflammation.

4. Antioxidant Activities of the Stilbenes and Propolis Extracts 1,1-Diphenyl-2-picrylhydrazyl (DPPH^(●)) Scavenging Activity Assay

The (1,1-diphenyl-2-picrylhydrazyl) DPPH assay is commonly used to test free radical scavenging ability of a compound or extracts of natural products by measurement of the reduction of DPPH^(●) radicals at 517 nm. In its radical form, DPPH^(●) shows a strong absorption at 517 nm due to its odd electron. Upon reduction by an antioxidant or radical scavenger, the absorption disappears and the resulting decolorization by scavenging the radical is stoichiometric with respect to the number of electrons taken up (DPPH^(●)+AH→DPPH:H+A^(●)). The DPPH assay was carried out in a stepwise procedure as described below.

A methanolic solution of DPPH (0.1 mM) was stirred in a dark container at room temperature for 20 min. The solution was scanned between 400-750 nm to obtain a maximum wavelength (λmax, ˜510 nm). The concentration of the DPPH solution was adjusted with methanol to result in a maximum absorbance of approximately 1.0. Test samples at different concentrations and standard antioxidant solution (0.05 mL) were added to 0.95 mL of methanolic DPPH solution in a cuvette. Final concentrations of the test samples were 0.1, 1, 10, 50, 100 and 200 μM. The mixtures were shaken vigorously and allowed to stand in the dark for 30 min at room temperature. Absorbance of the resulting solution was measured at the maximum wavelength (˜510 nm). A decrease in absorbance indicated a free radical scavenging effect of the test samples. Dose response curves of the test samples were established to determine their IC₅₀ values (concentrations that show 50% reduction in UV absorbance).

Results

pPHOS compounds in this study exhibited a moderate to weak effect on free radical scavenging except for USYDS7 which exhibited a strong effect. These results are displayed in FIG. 10.

5. Effect of Stilbene Derivatives on Nicotinamide Adenine Dinucleotide (NAD)-Dependent Deacetylase Sirtuin-2 (SIRT1)

SIRT1 is a member of Sir2 family (class III) which is a NAD-dependent histone deacetylase. Deacetylation by SIRT1 enzyme can target many substrates including histone, tumor suppressor p53, forkhead transcription factor (FOXO), peroxisome proliferator-activated receptor-γ (PPARγ) and co-activator-1α (PGC-1α).¹⁰ SIRT1 has been shown to be involved in the regulation of many physiopathological processes like inflammation, cellular aging, apoptosis/proliferation, metabolism and cell cycle regulation (Chung, Yao et al. 2010). Accordingly, modulating SIRT1 activity could be a potential therapeutic target to control many diseases such as cancer, metabolic syndrome, obesity, neurodegenerative disorder, skeletal muscle dysfunction and aging-related diseases.¹¹

SIRT1 assay kit (Cayman Chemical, Ann Arbor, Mich., USA) provides a fluorescence-based method for screening of SIRT1 inhibitors or activators. The assay was carried out according to the instructions from the manufacturer. In brief, the assay consists of two steps, both performed in the same plate. In the first step, the substrate, which comprises the p53 sequence Arg-His-Lys-Lys(ε-acetyl)-AMC (7-amino-4-methylcoumarin), is incubated with human recombinant SIRT1 along with its cosubstrate NAD⁺. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product which was analysed using fluorometric plate reader at an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm. Stilbenes were assayed at three concentrations (1, 10 and 100 μM). Data represents two independent experiments each performed in triplicate.

Results

All the stilbenes, except resveratrol, exhibited a concentration dependent inhibition of SIRT1 as shown in FIGS. 11 A and B. Modulation of SIRT1 activity could lead to the development of therapeutic agents for the treatment of diseases including cancer, metabolic syndrome, obesity, neurodegenerative disorder, and aging-related diseases.

6. Antibacterial Activities of USYDS1, USYDS2 and Ethanolic Extract of Sedge Type-1 Propolis Summary

The minimum inhibition concentration (MIC) screening was performed with 14 bacterial strains and 4 compounds. The MICs were determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The MIC screening was performed on 96-well plates containing the compounds in serial 2-fold dilutions from 64 to 0.06 μg/ml. The bacterial inoculations were prepared in cation-adjusted Mueller Hinton medium broth from cultures grown on appropriate agar plates which are prepared freshly every week. The growth controls and sterile controls were included in each assay plates. The assay plates were incubated in an ambient-air incubator at 35±2° C. for 16-20 hr (24 hr for MRSA), and bacterial growth was observed and recorded. All MICs of reference compound levofloxacin in the MIC screening are within the standard range described in CLSI S100-A20. The potency of 3 test samples is the order of USYDS1>USYDS2>ethanolic propolis extract.

1. Materials 1.1. Strains

Bacteria panel for MIC screening Cultivation MIC screening Microorganism Gram Strain ¹Resistance ²Plasmid condition condition Escherichia coli G⁻ ATCC TSA, ambient CAMHB, ambient (25922) air, 35 ± 2° C. air, 35 ± 2° C., 20 hr Pseudomonas G⁻ ATCC TSA, ambient CAMHB, ambient aeruginosa (27853) air, 35 ± 2° C. air, 35 ± 2° C., 20 hr Klebsiella G⁻ ATCC AMP, AZT, Yes TSA, ambient CAMHB, ambient pneumoniae (700603) CFX, CPD, air, 35 ± 2° C. air, 35 ± 2° C., 20 hr CAZ, CHL, PIP, TET Haemophilus G⁻ ATCC Chocolate agar, HTM, ambient influenzae (49247) 5% CO₂, air, 35 ± 2° C., 20 hr 35 ± 2° C. Acinetobacter G⁺ ATCC IMI TSA, ambient CAMHB, ambient calcoaceticus (51432) air, 35 ± 2° C. air, 35 ± 2° C., 20 hr Enterococcus G⁺ ATCC VAN TSA + 5% sheep CAMHB, ambient faecium (700221) blood, ambient air, 35 ± 2° C., 20 hr air, 35 ± 2° C. Enterococcus G⁺ ATCC TSA + 5% sheep CAMHB, ambient faecalis (29212) blood, ambient air, 35 ± 2° C., 20 hr air, 35 ± 2° C. Streptococcus G⁺ ATCC TSA + 5% sheep CAMHB + 3% horse pyogenes (700492) blood, 5% CO₂, blood, ambient air, 35 ± 2° C. 35 ± 2° C., 20 hr Streptococcus G⁺ ATCC PEN TSA + 5% sheep CAMHB + 3% horse pneumoniae (49619) blood, 5% CO₂, blood, ambient air, 35 ± 2° C. 35 ± 2° C., 20 hr Streptococcus G⁺ Clinical ERY TSA + 5% sheep CAMHB + 3% horse pneumoniae isolate blood, 5% CO₂, blood, ambient air, 35 ± 2° C. 35 ± 2° C., 20 hr Staphylococcus G⁺ ATCC TSA, ambient CAMHB, ambient aureus (29213) air, 35 ± 2° C. air, 35 ± 2° C., 20 hr Staphylococcus G⁺ ATCC MET, OXA TSA, ambient CAMHB, ambient aureus (43300) air, 35 ± 2° C. air, 35 ± 2° C., 20 hr Staphylococcus G⁺ Clinical LEV TSA, ambient CAMHB, ambient aureus isolate air, 35 ± 2° C. air, 35 ± 2° C., 20 hr Staphylococcus G⁺ Clinical MET, ERY, TSA, ambient CAMHB, ambient aureus isolate CLI air, 35 ± 2° C. air, 35 ± 2° C., 20 hr ¹Known resistance; ²Known plasmid presence Abbreviation: TSA, trypticase soy agar; CAMHB, cation-adjusted Mueller Hinton broth; HTM, Haemophilus test medium; AMP, ampicillin; AZT, aztreonam; CFX, cefoxitin; CPD, cefpodoxime; CAZ, ceftazidime; CHL, chloramphenicol; PIP, piperacillin; TET, tetracycline; IMI, imipenem; VAN, vancomycin; PEN, penicillin; ERY, erythromycin; MET, methicillin; OXA, oxacillin; LEV, levofloxacin; CLI, clindamycin.

1.2. Media and Reagents

Trypticase soy agar (BD 211043), Cation-adjusted Mueller Hinton broth (BD 212322), Haemophilus test medium base (Fluka 51295), Hemin (Fluka 51280), β-NAD (Fluka 43410), Levofloxacin (Sigma 28266), Sheep blood (Quad Five 630-500), Lysed horse blood (Quad Five 205-500), 0.5 McFarland barium sulfate standard, Sterile 0.85% NaCl (w/v).

2. Methods 2.1. Prepare Bacterial Strains

A. Revive bacterial strains from storage frozen (−80° C.) two days before the MIC screening. Streak onto surface of appropriate agar plates, and incubate the plates for 20-24 hr at 35±2° C. in an appropriate atmosphere.

Streptococci: TSA II, 5% CO₂

Enterococci: TSA II, ambient air

Haemophilus influenzae: chocolate agar, 5% CO₂

Other strains in the panel: TSA, ambient air

B. Select 5-10 well-isolated colonies of similar morphology and restreak onto fresh agar plates using sterile loops. Incubate the plates for 20-24 hr at 35±2° C. in an appropriate atmosphere as above.

2.2. Prepare Compound Plates

Compound stock solutions were prepared in 100% DMSO on the day of MIC screening and use immediately. Compound stock concentration=[(highest testing concentration) ×103 μl/3 μl] (e.g. if the required highest testing concentration is 64 μg/ml in assay plates, stock concentration=64×103/3=2.2 mg/ml). The potency of testing compound is assumed as 100% unless otherwise stated whereas the potency of reference compound is calculated according to manufacturer's analysis data.

Eleven two-fold dilutions per compound were made then transferred 3 μl to each well of test plate. Final concentration of DMSO in the MIC screening is ˜3%.

2.3. Prepare Bacterial Inoculation

A. Take out medium broth from 4° C. fridge and allow it to warm to room temperature.

B. Transfer colonies from fresh culture plates into 5 ml of saline with sterile loops and mix well. Measure and adjust turbidity to 0.5 McFarland barium sulphate standard using a turbidity meter. Alternatively, transfer 1-2 colonies into 500 μl of saline and adjust OD625 to ˜0.1 using a plate reader.

C. Dilute bacterial inoculum 1:280 for Gram-positive and fastidious strains and 1:400 for Gram-negative strains into corresponding medium broth (CAMHB, CAMHB+3% lysed horse blood, HTM) (e.g. 35.6 μl of inoculum into 10 ml of CAMHB or 25 μl of inoculum into 10 ml of CAMHB).

H. influenzae: HTM

Streptococci: CAMHB+3% lysed horse blood

Other strains in the panel: CAMHB

2.4. Prepare Assay Plates

A. Add 100 μl of the bacterial inoculum to each well of the compound plates except wells B12, D12, F12 and H12.

B. Add 100 μl of medium broth to wells B12, D12, F12 and H12 of the compound plates.

C. Stack four plates together and cover with a sterile plate lid. Incubate in an ambient-air incubator at 35±2° C. for 16-20 hours (24 hours for MRSA).

2.5.. Perform Colony Counts

A. Dilute the bacterial inoculum (0.5 McFarland) to a serial of 10−1 to 10−7 in saline solution (e.g. 100 μl bacterial inoculum+900 μl of saline).

B. Spread 100 μl of each dilution (10−4, 10−5, 10−6, and 10−7) onto CAMHA plates in triplication, let the liquid soak into the agar for 10 minutes, invert the agar plates and incubate for 24 hr at 35±2° C.

2.6. Record MICs and Calculate CFUs

A. Open the compound plate layout in the compound management system, and check the assay plate barcode.

B. Place the assay plate on the top of MIC reader, and adjust the magnification mirror to read each wells, record growth status as raw data. (Optional) Record photo image of each assay plates using high-speed high-resolution scanner.

C. Determine MIC break points according to CLSI guideline.

D. Count colonies and calculate CFU of bacterial inoculum.

3. Results 3.1. MIC Summary Table

The MIC screening was performed with 14 bacterial strains (11 ATCC strains and 3 clinical isolates) and 4 compounds (USYDS1, USYDS2 and ethanolic extract of propolis and reference compound levofloxacin). The MICs are summarized in the below Table 6. The MIC values of reference compound Levofloxacin obtained in this study are within the standard range as described in S100-A20 [2]. The final concentration of DMSO in the MIC screening was ˜3%, and did not inhibit the growth of most microorganisms.

TABLE 6 MICs (μg/ml) of USYDS1, USYDS2 and ethanolic extract of propolis against fourteen bacterial strains. Levofloxacin is reference compound. Ethanolic propolis Levofloxacin USYDS1 USYDS2 extract Compounds Exp 1 Exp 2 Exp 1 Exp 2 Exp 1 Exp 2 Exp 1 Exp 2 Acinetobacter calcoaceticus 4 4 >64 >64 >64 >64 >64 >64 Escherichia coli <0.0625 <0.0625 16 16 64 32 >64 >64 Enterococcus faecalis 1 1 16 16 32 32 64 64 (ATCC 29212) Enterococcus faecium >64 >64 8 16 32 32 64 32 (ATCC 700221) Haemophilus influenzae <0.0625 <0.0625 32 32 32 32 64 64 Klebsiella pneumoniae 1 1 >64 >64 >64 >64 >64 >64 Pseudomonas aeruginosa 1 1 >64 >64 >64 >64 >64 >64 Staphylococcus aureus 0.5 0.25 16 8 32 16 32 32 (ATCC 29213) Staphylococcus aureus 0.25 0.25 8 8 16 16 32 32 (ATCC 43300) Staphylococcus aureus 64 64 8 8 16 16 16 16 (Levofloxacin-resistant) Staphylococcus aureus 8 8 8 8 16 16 32 32 (MRSA, Erythromycin & clindamycin-resistant) Streptococcus pneumoniae 0.5 0.5 32 32 32 32 32 32 (ATCC 49619) Streptococcus pyogenes 0.5 0.5 64 64 64 64 >64 >64 (ATCC 700942) Streptococcus pneumoniae 1 1 32 32 32 64 64 >64 (Erythromycin-resistant)

4. Discussion

The prenylated tetrahydroxystilbenes USYDS1 and USYDS2 showed a moderate anti-bacterial activities with the rank of potency of USYDS1>USYDS2>ethanolic extract of propolis.

7. The Effect of USYDS1, USYDS2 and USYDS10 Compounds of Kinases Activities

The following is a list of kinases in the studied.

No. Kinase 1 ABL2 2 AKT1 3 AKT2 4 AKT3 5 ALK1 6 AMPK(A1/B1/G1) 7 AMPK(A2/B1/G1) 8 Aurora A 9 Aurora B 10 Aurora C 11 AXL 12 BLK 13 BTK 14 CAMK1 15 CDK1/CyclinA2 16 CDK1/CyclinB 17 CDK2/CyclinA2 18 CDK3/CyclinE1 19 CDK4/CyclinD1 20 CDK4/CyclinD3 21 CDK5/CyclinP25 22 CDK6/CyclinD1 23 CDK6/CyclinD3 24 CDK7/CyclinH1/MNAT1 25 CHK1 26 c-KIT 27 c-KIT(V654A) 28 EGFR(T790M, L858R) 29 EphA1 30 EphA2 31 EphA3 32 EphA4 33 EphB1 34 EphB2 35 EphB3 36 ERK1 37 FGFR1(V561M) 38 FGFR2 39 FGFR3 40 FGR 41 FLT1(VEGFR1) 42 FLT3 43 GSK3α 44 HER2 45 IGF1R 46 InsR 47 KDR 48 LCK 49 NEK2 50 p38β 51 PDGFRβ 52 PI3Kα 53 PI3Kβ 54 PI3Kγ 55 PI3Kδ 56 PKAcα 57 PKAcβ 58 PKAcγ 59 PKCα 60 PKCβI 61 PKCγ 62 PKCε 63 PKCθ 64 PKCδ 65 PKCδ 66 PKCι 67 PLK2 68 PLK3 69 RAF1 70 BRAF 71 BRAF(v599E) 72 RET 73 RON 74 SRC 75 TrkA 76 TrkB

Experiments Materials

Kinase-Glo(Plus)/ADP-Gloassay buffer

25 mMHEPES, 10 mMMgCl2, 0.01% Triton X-100, 100 μg/mLBSA, 2.5 mMDTT, pH 7.4.

Caliper assay buffer

100 mMHEPES, 10 mMMgCl2, 100 μl/L Brij35 (30%), 1 mMDTT, pH 7.4.

Assay Substrates

MBP protein, UnactiveMEK1, Rbprotein were purchased from SignalChem. Poly(glu:tyr)(4:1) was purchased from Sigma. PIP2 was purchased from Cayman. Peptide substrates were synthesized in HD Biosciences, China.

ATP was purchased from Sigma. KinaseGloPlus reagent, KinaseGloreagent and ADP Gloreagent were purchased from Promega

Assay Procedure—Caliper Format

Mix Kinases, substrate, ATP and compound in 96-well assay plate, total volume is 50 μL. Incubate assay plate at 30° C. for 1 hour. Stop reaction by adding 20 μL of 35 mM EDTA and transfer 26 μL stopped reaction to 384-well assay plate. Read the assay plate on the plate reader.

Assay Procedure—ADP-GloFormat

Mix Kinases, substrate, ATP and compound in 384-well assay plate, total volume is 10 μl. Incubate assay plate at 30° C. for 1 hour. Add 10 μl/well of ADP GloReagent to the assay plate, incubate at 27° C. for 40 min.

Add 20 μl/well Detection Reagent to the assay plate, incubate at 27° C. for 30 min. Read the assay plate on the plate reader

Assay Procedure—Kinase-Glo(Plus)Format

Mix Kinase, substrate, ATP and compound in 384-well assay plate, total volume is 10 μl. Incubate assay plate at 30° C. for 1 hour. Add 10 μl/well KinaseGlo(Plus) reagent to the reaction mixture, and then incubate at 27° C. for 20 min. Read the assay plate on plate reader.

Hundred percent effect was performed without compound and enzyme, but containing ATP and substrate. Zero percent effect was carried out without compound, but containing ATP, substrate and enzyme. SB202190 is reference compounds for kinase p38β; Staurosporine (STSP) is reference compound for the remaining kinases.

Results

Kinases that inhibited more than 60% are summarised in the table below. It is interesting to note that all three compounds inhibited kinases TrKA and PI3Kδ and PI3Kγ. Both USYDS1 and USYDS10 appear to display similar inhibitory activities towards the kinases.

Compound Kinases USYDS1 FLT3, TrkA, CDK2/CyclinA2, CDK4/CyclinD3, PI3Kα, PI3Kδ, PI3Kγ USYDS2 TrkA, PI3Kβ, PI3Kδ, PI3Kγ USYDS10 FLT3, TrkA, KDR, FLT1, CDK2/CyclinA2, PI3Kα, PI3Kβ, PI3Kδ, PI3Kγ, CDK1/CyclinB

8. Acute Toxicity Study of USYDS1 Method

A single mouse is given a single IP injection of 400 mg/kg; a second mouse receives a dose of 200 mg/kg IP and a third mouse receives a single dose of 100 mg/kg IP. The mice are observed for a period of 2 weeks. They are sacrificed if they lose more than 20% of their body weight or if there are other signs of significant toxicity. If all 3 mice must be sacrificed, then the next 3 dose levels (50, 25, 12.5 mg/kg) are tested in a similar way. This process is repeated until a tolerated dose is found. This dose is then designated the maximum tolerated dose (MTD) and is used to calculate the amount of material given to experimental mice during anti-tumor testing. The mice are allowed ad libitum feed and water. Drug was dissolved in 100% DMSO at concentration of 200 mg/mL.

Result

Dose Death Survivor/ Group (mg/kg/dose) Route days Total day 15 Injection volume 1 100 IP None 1/1 0.5 μL/gm body wt 2 200 IP 1 0/1 1 μL/gm body wt 3 400 IP 1 0/1 2 μL/gm body wt

The MTD of USYDS1 was determined as 100 mg/kg. This concentration indicates low mammalian toxicity and is being used for further anti-tumor testing. Hollow fiber assay (BEC/C) for USYDS1 is under progression. The hollow fibre assay is a preliminary rapid screen for assessing novel putative chemotherapeutic compounds against a range of cancer cell lines prior to their evaluation in the mouse xenograft model. The hollow fiber model has a shorter evaluation time and a reduced compound requirement compared to traditional xenograft models. The model allows for the effective selection of cancer cell types in the xenograft.

Chemotypes of Plants that Produce Resins as Sources of Prenylated Polyhydroxystilbene Derivatives

Resins, gums or exudates obtained from plants of the Lepidosperma genus from different locations were analysed by quantitative ¹H-NMR (q-NMR) for prenylated polyhydroxystilbene content, which include C- and O-prenylated, O-methylated and non-O-methylated derivatives. Different proportions of these prenylated polyhydroxystilbene derivatives from the resins form a basis to classify the plants accordingly.

There are at least 3 different chemotypes of Lepidosperma plants identified thus far and each of these plants display several sub-chemotypes. Type 1 is the most common plant which contains approximately equal proportion of both C- and O-prenylated derivatives. Type 2 plant contains only C-prenylated derivatives. Where as, type 3 plant contains no O-methylated prenylated polyhydroxystilbene derivatives.

It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.

REFERENCES

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(5) Andrus, M. B.; Liu, J.; Meredith, E. L.; Nartey, E. Tetrahedron Lett. 2003, 44, 4819-4822.

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What is claimed is:
 1. A compound according to formula (Ia):

or a pharmaceutically acceptable sgalt or solvate thereof, wherein: R^(1a) is selected from CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; R^(1b) is selected from, H, OH, OR⁴, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; R^(1c) is selected from H, OH, OR^(4a), CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; R^(1d) is selected from H, OH, CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; wherein at least one of R^(1b-1d) is OH and at least one of R^(1a-1d) is CH₂CH═C(CH₃)₂, OCH₂CH═C(CH₃)₂, CH═CHCH(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; R² is selected from OH, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; R³ is selected from OH, OR⁴, OCH₂CH═C(CH₃)₂, CH═CHC(CH₃)═CH₂, OCH═CHCH(CH₃)₂, or OCH═CHC(CH₃)═CH₂; R⁴ is selected from, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl or benzyl; and A----B is selected from CH═CH, CH₂CH₂CH₂, CH═CHX, or CH₂—CH₂X, where X═(CH₂)pCH₂ and p is an integer selected from the group consisting of 1 or 2, wherein the compound originates from propolis of the Lepidosperma genus.
 2. A composition comprising a compound of claim 1 together with an acceptable excipient.
 3. A method of treating inflammation, immunosuppression, skin ageing, anti-bacterial or antifungal infection, comprising administering a compound according to claim 1 or a composition according to claim 2 to a subject in need thereof. 